Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, PR China.
The Life Science College, Hebei Normal University, Shijiazhuang 050024, PR China.
Bioorg Chem. 2021 Jul;112:104984. doi: 10.1016/j.bioorg.2021.104984. Epub 2021 May 11.
Wild mushrooms are an important source of secondary metabolites possessing a broad range of biological activities. In this study, eight new compounds, named furanopaxin A-F (1-6), deoxybisinvolutone (7), and coumarinvol (8) along with two known compounds were isolated from fruiting bodies of the wild mushroom Paxillus involutus (Batsch) Fr. Their structures were established based on HR-ESI-MS and 1D and 2D NMR spectroscopic data. The results of hypoglycemic assays indicated that compounds 5-8 possessed significant α-glucosidase inhibitory activities, with IC values ranging from 14.65 ± 1.68 to 47.55 ± 1.47 μM, and each compound could enhance glucose consumption in insulin-resistance HepG2 cells. Further analysis by molecular docking implied that compounds 5-8 could interact with the amino acid residues of α-glucosidase, supporting the hypoglycemic activity of the compounds.
野生蘑菇是次生代谢产物的重要来源，具有广泛的生物活性。本研究从野生蘑菇鲍氏鳞伞（Paxillus involutus）（Batsch）Fr 的子实体中分离得到了 8 个新化合物，分别命名为呋喃帕克斯辛 A-F（1-6）、脱氧比辛诺酮（7）和香豆素伏洛（8），以及 2 个已知化合物。基于高分辨电喷雾质谱（HR-ESI-MS）和 1D 和 2D NMR 波谱数据确定了它们的结构。降血糖实验结果表明，化合物 5-8 对 α-葡萄糖苷酶具有显著的抑制活性，IC 值范围为 14.65±1.68 至 47.55±1.47μM，且每种化合物均可促进胰岛素抵抗 HepG2 细胞中的葡萄糖消耗。分子对接进一步分析表明，化合物 5-8 可与 α-葡萄糖苷酶的氨基酸残基相互作用，支持化合物的降血糖活性。
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