[利用交叉放射免疫电泳和放射变应原吸附试验抑制法对艾蒿花粉（普通蒿）进行变应原分析]
[Allergen analysis of mugwort pollen (Artemisia vulgaris) using crossed radioimmunoelectrophoresis and radioallergosorbent test inhibition].
作者信息
Diener C, Frenzel K, Wiebicke K, Schlenvoigt G, Fuster R, Jäger L
机构信息
Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
出版信息
Allerg Immunol (Leipz). 1988;34(1):3-9.
It could be identified 43 antigens of mugwort pollen (Artemisia vulgaris), 31 migrating anodically and 12 cathodically, by means of crossed immunoelectrophoresis (CIE). Eleven antigens bound human IgE-antibodies in crossed radioimmunoelectrophoresis (CRIE). Two major allergens could be found, allergens 23 and 40.- The allergenic activity of mugwort pollen extract was examined by means of RAST-inhibition. Specificity of this reaction could be determined by a control trial with grass pollen extract.
通过交叉免疫电泳（CIE）可鉴定出艾蒿花粉（艾蒿）的43种抗原，其中31种向阳极迁移，12种向阴极迁移。在交叉放射免疫电泳（CRIE）中，11种抗原与人IgE抗体结合。可发现两种主要过敏原，即过敏原23和40。通过RAST抑制试验检测了艾蒿花粉提取物的致敏活性。该反应的特异性可通过用草花粉提取物进行对照试验来确定。
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