Water Research Institute, Shanghai Academy of Environmental Sciences, Shanghai, People's Republic of China.
Environ Technol. 2022 Sep;43(23):3608-3619. doi: 10.1080/09593330.2021.1928295. Epub 2021 May 23.
The performance of photo-sequencing batch reactor (PSBR) in removing multiple antibiotics and nutrients from aquaculture wastewater as well as the antibiotic resistance genes (ARGs) proliferation were firstly investigated during the long-term experiments. The operational conditions (i.e. light intensity, light time, aeration and solid retention time) were optimised to realise the simultaneous removal of antibiotics and nutrients. It was found that, compared with traditional SBR, PSBR has similar nutrient removal rate and a 30% higher antibiotics removal rate due to the corporation of microalgae () and bacteria, and the absolute abundance of ARGs decreased by 78% in PSBR. Further investigation showed that PSBR had certain advantages in removing quinolones and the corresponding removal rate could reach up to 90%. In terms of the mechanisms, the possible metabolic pathway of antibiotic was analysed and the intermediate metabolites were different from that of the reported studies. The microbial communities were also affected by microalgae and the relative abundance of certain bacteria (such as members of the families and ), which were positively correlated with some ARGs, decreased in PSBR. This study provides an alternative and effective method to aquaculture wastewater treatment, which present high nutrients and antibiotics removal efficiencies and low ARGs transmission.
本研究首次采用光序批式反应器（PSBR）长期运行，考察其去除养殖废水中多种抗生素和营养物以及抗生素抗性基因（ARGs）增殖的性能，并优化了运行条件（光照强度、光照时间、曝气和固体停留时间）以实现抗生素和营养物的同步去除。结果表明，与传统序批式反应器（SBR）相比，由于微藻（）和细菌的共同作用，PSBR 具有相似的营养物去除率和 30%更高的抗生素去除率，且 PSBR 中 ARGs 的绝对丰度降低了 78%。进一步的研究表明，PSBR 对喹诺酮类抗生素具有一定的去除优势，其相应的去除率可高达 90%。就作用机制而言，分析了抗生素的可能代谢途径，且中间代谢产物与报道的研究不同。微藻还影响了微生物群落，与某些 ARGs 呈正相关的某些细菌（如 和 科的成员）的相对丰度在 PSBR 中降低。本研究为养殖废水处理提供了一种替代且有效的方法，该方法具有高效的去除营养物和抗生素以及低的 ARGs 传播风险。
