Zhang J Y, Sun Z G, Li M, Yang Y R, Huang J A, Liu J X
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1989;7(3):197-200.
The Avidin-Biotin-Peroxidase Complex enzyme-linked immunosorbent assay (ABC-ELISA) with antigen from gerbils infected with alveolar hydatid (GAH) was performed on the sera of 54 patients with multilocular echinococcosis, 107 patients with other diseases and 102 healthy adults. All the sera were diluted 1:200 and an S/N value greater than 2.2 was taken as positive. Both the sensitivity and specificity were 98.1%. The S/N value (mean +/- SD) of sera of the patients with multilocular echinococcosis, other parasitic diseases, tuberculosis, other diseases and healthy adults were: 5.9 +/- 3.0, 1.1 +/- 0.4, 1.4 +/- 0.8, 1.1 +/- 0.5 and 1.0 +/- 0.4. The geometric mean titre (GMT) of antibody measured by ABC-ELISA was 13.7 times that of ELISA. The quantity of serum used in ELISA was 8 times that of ABC-ELISA. ABC-ELISA might be particularly helpful to identify cases with low immune responsiveness. In the detection of alveolar hydatidosis and cystic disease with GAH antigens, the positivity was 98.1 and 67.1% with 87 and 25.6% having S/N greater than 5, respectively. It is suggested that in the diagnosis of hydatid diseases by ABC-ELISA, homologous antigen should be used. The temperature and the incubation time needed for the present assay are practical, for the total time requires only 55 minutes.
采用感染肺泡型包虫的沙鼠抗原（GAH）的抗生物素蛋白-生物素-过氧化物酶复合物酶联免疫吸附测定（ABC-ELISA），对54例泡型棘球蚴病患者、107例其他疾病患者和102名健康成年人的血清进行检测。所有血清均稀释1:200，S/N值大于2.2为阳性。敏感性和特异性均为98.1%。泡型棘球蚴病患者、其他寄生虫病患者、结核病患者、其他疾病患者和健康成年人血清的S/N值（平均值±标准差）分别为：5.9±3.0、1.1±0.4、1.4±0.8、1.1±0.5和1.0±0.4。ABC-ELISA法检测的抗体几何平均滴度（GMT）是ELISA法的13.7倍。ELISA法所用血清量是ABC-ELISA法的8倍。ABC-ELISA法可能对识别免疫反应性低的病例特别有帮助。在用GAH抗原检测肺泡型包虫病和囊型包虫病时，阳性率分别为98.1%和67.1%，S/N大于5的分别为87%和25.6%。建议在ABC-ELISA法诊断包虫病时应使用同源抗原。本检测所需的温度和孵育时间切实可行，因为总时间仅需55分钟。
