Ye Y Y, Zhao S, Liu Y H, Bi N N, Dong X, Xiong C R, Zhu H R, Tang F, Wang X Y, Zhang J F, Ying Q J, Yang K
National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
Jiangsu Qitian Gene Technology Co., Ltd., China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2021 Apr 8;33(2):185-188. doi: 10.16250/j.32.1374.2020281.
To evaluate the efficiency of a recombinase-aided amplification (RAA) assay for the detection of infections in snails.
A group test was employed. Fifty snails were collected as a detection sample. The detection samples without infected snails were designated as negative specimens, while the detection samples that contained different numbers of infected snails were designated as positive specimens. A total of 10 negative specimens, 10 positive specimens containing 1 infected snail, 20 positive specimens containing 2 infected snails and 10 positive specimens containing 3 infected snails were assigned. Following random grouping, 40 specimens were subject to the florescent RAA assay using a blind method. The miradium shedding method served as a gold standard, and the sensitivity, specificity, Youden's index and coincidence rate of the florescent RAA assay were estimated. In addition, 20 samples consisted of 5 negative specimens and 15 positive specimens with 1, 2 and 3 infected snails respectively were grouped randomly. The same specimens were detected using the crushing method and fluorescent RAA assay with the blind method in a paired-design manner. Then, the test results were compared and analyzed.
Florescent RAA assay detected 29 positives in the 30 specimens containing different numbers of infected snails, with a sensitivity of 96.67%, and 8 negatives in the 10 detection specimens without infected snails, with a specificity of 80.00%, showing a Youden's index of 0.77. The coincidence rate was 100% among 10 repeated assays for a detection specimen. In addition, there was no significant difference in the detection of infected snails between the florescent RAA assay and the crushing method ( = 0, > 0.05), and the actual coincidence rates of the florescent RAA assay and crushing method were 95.00% (19/20) and 90.00% (18/20) with the real results, respectively.
Fluorescent RAA assay has a favorable efficiency for the detection of infections in snails, which shows a potential in screening of -infected snails.
评估重组酶辅助扩增（RAA）检测法用于检测钉螺感染的效率。
采用分组检测。收集50只钉螺作为检测样本。未感染钉螺的检测样本被指定为阴性标本，而含有不同数量感染钉螺的检测样本被指定为阳性标本。共分配10份阴性标本、10份含1只感染钉螺的阳性标本、20份含2只感染钉螺的阳性标本和10份含3只感染钉螺的阳性标本。随机分组后，40份标本采用盲法进行荧光RAA检测。以miracidium逸出法作为金标准，估算荧光RAA检测法的灵敏度、特异性、约登指数和约合率。此外，将20份样本随机分组，其中包括5份阴性标本和15份分别含1只、2只和3只感染钉螺的阳性标本。采用配对设计，对相同标本分别用碾碎法和荧光RAA检测法进行盲法检测。然后，对检测结果进行比较和分析。
荧光RAA检测法在30份含有不同数量感染钉螺的标本中检测出29份阳性，灵敏度为96.67%，在10份未感染钉螺的检测标本中检测出8份阴性，特异性为80.00%，约登指数为0.77。对一份检测标本进行10次重复检测，约合率为100%。此外，荧光RAA检测法与碾碎法在检测感染钉螺方面无显著差异（=0，>0.05），荧光RAA检测法和碾碎法与实际结果的实际约合率分别为95.00%（19/20）和90.00%（18/20）。
荧光RAA检测法在检测钉螺感染方面具有良好的效率，在筛查感染钉螺方面显示出潜力。
