Derksen F J, Robinson N E, Scott J S, Stick J A
Pulmonary Laboratory, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
Am J Vet Res. 1988 Jun;49(6):933-8.
Ponies with recurrent airway obstruction (principal ponies) and their controls were given aerosolized Micropolyspora faeni antigen via endotracheal tube during a period when the principal ponies were in disease remission. In both groups of ponies, we performed bronchoalveolar lavage (BAL) and measured pulmonary function at base line, and 5 hours after aerosol administration of 30 ml of 0.9% NaCl solution or 30 ml of 1% w/v particulate M faeni antigen in 0.9% NaCl solution. In both groups of ponies, aerosolized M faeni antigen increased WBC count, neutrophil numbers, and albumin concentration in BAL fluid, but macrophage numbers decreased. In the principal ponies, BAL mast cell numbers were decreased 5 hours after administration of M faeni antigen. The M faeni antigen had no effect on the mechanical properties of the lungs or on gas exchange in the control ponies, but did increase respiratory frequency minute ventilation and pulmonary resistance, and decreased arterial oxygen tension in the principal ponies. Changes in pulmonary function were apparent only in the principal ponies, which suggests that neutrophils, per se, do not cause pulmonary dysfunction and that M faeni may be one of the etiologic agents involved in chronic obstructive pulmonary disease.
在复发性气道阻塞的矮种马（主要矮种马）及其对照处于疾病缓解期时，通过气管内导管给它们雾化吸入微小多孢菌抗原。在两组矮种马中，我们在基线时以及雾化给予30毫升0.9%氯化钠溶液或30毫升含1%重量/体积微小多孢菌抗原的0.9%氯化钠溶液5小时后，进行支气管肺泡灌洗（BAL）并测量肺功能。在两组矮种马中，雾化吸入微小多孢菌抗原均增加了BAL液中的白细胞计数、中性粒细胞数量和白蛋白浓度，但巨噬细胞数量减少。在主要矮种马中，给予微小多孢菌抗原5小时后，BAL肥大细胞数量减少。微小多孢菌抗原对对照矮种马的肺力学特性或气体交换没有影响，但在主要矮种马中确实增加了呼吸频率、分钟通气量和肺阻力，并降低了动脉血氧张力。肺功能变化仅在主要矮种马中明显，这表明中性粒细胞本身不会导致肺功能障碍，并且微小多孢菌可能是慢性阻塞性肺疾病的病因之一。
