Department of Plant Breeding and Genetics, Anbil Dharmalingam Agricultural College and Research Institute, Tamil Nadu Agricultural University, Tiruchirappalli, Tamil Nadu, India.
Department of Vegetable Science, Horticultural College and Research Institute for Women, Tamil Nadu Agricultural University, Tiruchirappalli, Tamil Nadu, India.
Methods Mol Biol. 2021;2290:129-138. doi: 10.1007/978-1-0716-1323-8_9.
Biofuels offer a solution to combat climate change and secure energy surplus. Among the various sources for biodiesel production, the seed of oilseed crops is primary raw material. The biodiesel industry demands a supply of good quality oil so that it can reduce processing costs and price of biodiesel. In general, the oils with low free fatty acid contents are considered as good quality oil and for which the quality of the oil should be analyzed in seed lot for biodiesel production. Currently, oil quality testing is done to the oil extracted from seed lot using sophisticated instruments. However, the analysis of oil seed lot is not tested, which forms the basic materials for oil extraction. Therefore, analysis of the quality of raw materials (seed lot in this case) is mandatory for assessing oil quality.Till now, there is no standard method available for screening the quality of the oilseed before biodiesel production. The free fatty acid (FFA) content of the oil is the critical factor for biodiesel production. In seeds, the FFA content of oil is directly associated with seed viability. In this chapter, an easy-to-perform protocol is presented for determining the seed viability potential, which is having a significant positive association with FFA content of the seed.
生物燃料为应对气候变化和保障能源过剩提供了一种解决方案。在生物柴油生产的各种原料中，油料作物的种子是主要的原料。生物柴油产业需要供应高质量的油，这样才能降低加工成本和生物柴油的价格。一般来说，低游离脂肪酸含量的油被认为是高质量的油，因此应该在用于生产生物柴油的种子批中分析油的质量。目前，使用精密仪器对从种子批中提取的油进行油质检测。然而，对形成油提取基础材料的油籽批本身却没有进行质量分析。因此，对原材料（在这种情况下是种子批）的质量进行分析是评估油质的强制性要求。到目前为止，在进行生物柴油生产之前，还没有一种标准的方法可以用来筛选油料的质量。油中的游离脂肪酸（FFA）含量是生物柴油生产的关键因素。在种子中，油的 FFA 含量直接与种子的活力相关。在本章中，提出了一种易于执行的方案来确定种子活力的潜力，该方案与种子的 FFA 含量具有显著的正相关性。
