Institute of Microbial Engineering, Laboratory of Bioresource and Applied Microbiology, School of Life Sciences, Henan University, Kaifeng, China.
Engineering Research Center for Applied Microbiology of Henan Province, Kaifeng, China.
Biotechnol Appl Biochem. 2022 Jun;69(3):1190-1198. doi: 10.1002/bab.2195. Epub 2021 May 31.
Glycerol is an abundant byproduct of biodiesel production that has significant industrial value and can be converted into dihydroxyacetone (DHA). DHA is widely used for the production of various chemicals, pharmaceuticals, and food additives. Gluconobacter can convert glycerol to DHA through two different pathways, including membrane-bound dehydrogenases with pyrroloquinoline quinone (PQQ) and NAD(P) -dependent enzymes. Previous work has indicated that membrane-bound dehydrogenases are present in Gluconobacter oxydans and Gluconobacter frateurii, but the metabolic mechanism of Gluconobacter thailandicus's glycerol conversion is still not clear. Through in-depth analysis of the G. thailandicus genome and annotation of its metabolic pathways, we revealed the existence of both PQQ and NAD(P) -dependent enzymes in G. thailandicus. In addition, this study provides important information related to the tricarboxylic acid cycle, glycerol dehydrogenase level, and phylogenetic relationships of this important species.
甘油是生物柴油生产的一种丰富的副产物，具有重要的工业价值，可以转化为二羟丙酮（DHA）。DHA 广泛用于生产各种化学品、药品和食品添加剂。葡糖杆菌可以通过两种不同的途径将甘油转化为 DHA，包括带有吡咯喹啉醌（PQQ）的膜结合脱氢酶和 NAD（P）依赖性酶。以前的工作表明，膜结合脱氢酶存在于氧化葡糖杆菌和弗氏葡糖杆菌中，但泰国葡糖杆菌甘油转化的代谢机制尚不清楚。通过对 G. thailandicus 基因组的深入分析和对其代谢途径的注释，我们揭示了 PQQ 和 NAD（P）依赖性酶在 G. thailandicus 中的存在。此外，本研究还提供了与三羧酸循环、甘油脱氢酶水平以及该重要物种的系统发育关系相关的重要信息。
