College of Chemistry, Sichuan University, Chengdu, China.
Analytical & Testing Center, Sichuan University, Chengdu, Sichuan, China.
Luminescence. 2021 Sep;36(6):1483-1490. doi: 10.1002/bio.4087. Epub 2021 Jun 2.
Using the principle of binding-induced DNA strand displacement (BINSD), a DNAzyme-powered nanomachine biosensor for multiple biomarkers via magnetic beads-based signal conversion was designed. This sensor can convert multiple biomarker recognition into release of predesigned output nucleic acids tagged with streptavidin proteins (SA-DNA) for activation of DNA nanomachines. In general, we adopted complementary base pairing rules and affinity ligand specific recognition, and three types of signal conversion systems were constructed that realized universal, sensitive, accurate, and specific detection of multiple biomarkers. Taking the advantage of the strong anti-interference capability of magnetic separation, this strategy could be used for detection of various biomarkers in clinical practice.
基于结合诱导的 DNA 链置换（BINSD）原理，设计了一种通过基于磁珠的信号转换的 DNA 酶驱动纳米机器生物传感器用于多种生物标志物的检测。该传感器可以将多种生物标志物识别转化为释放带有链霉亲和素蛋白的预定设计输出核酸（SA-DNA），以激活 DNA 纳米机器。通常，我们采用互补碱基配对规则和亲和配体特异性识别，并构建了三种信号转换系统，实现了多种生物标志物的通用、敏感、准确和特异性检测。利用磁分离的强抗干扰能力，该策略可用于临床实践中各种生物标志物的检测。
