Jackson P J, Sampson C J, Cooper E H, Heney D, Brocklebank J T
Unit for Cancer Research, University of Leeds, UK.
Ann Clin Biochem. 1988 May;25 ( Pt 3):319-24. doi: 10.1177/000456328802500322.
We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.
我们描述了一项使用Pharmacia PhastSystemTM电泳仪对蛋白尿进行的研究。通过对未浓缩尿液进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE），随后进行银染色来分析尿蛋白，这一过程耗时约2小时，对于蛋白试纸检测呈阴性或仅微量阳性的尿液，能够清晰地显示肾小管功能障碍或肾小球损伤。此外，我们展示了通过SDS-PAGE凝胶免疫印迹法鉴定尿蛋白，以及通过等电聚焦（IEF）和免疫印迹法对本-周蛋白进行表征。
