Molecular Characterization of All-Stage and Adult-Plant Resistance Loci Against Powdery Mildew in Winter Wheat Cultivar Liangxing 99 Using BSR-Seq Technology.

Winter wheat cultivar Liangxing 99, which carries gene , is resistant to powdery mildew at both seedling and adult-plant stages. An F recombinant inbred line population from cross Liangxing 99 × Zhongzuo 9504 was phenotyped with f. sp. isolate at the adult-plant stage in four field tests and the seedling stage in a greenhouse test. The analysis of bulk segregant RNA sequencing (BSR-Seq) identified a single-nucleotide polymorphism-enriched locus, , on chromosome 2BL in the same genomic interval of associated with the all-stage resistance (ASR) and on chromosome 7AL associated with the adult-plant resistance (APR) against the disease. was detected in a 1.3 cM genetic interval between markers and in which was placed with a range of logarithm of odds (LOD) values from 28.1 to 34.6, and the phenotype variations explained in terms of maximum disease severity (MDS) ranged from 45 to 52%. The LOD peak of was localized in a 4.6 cM interval between markers and and explained the phenotypic variation of MDS ranging from 13 to 16%. The results of this study confirmed for ASR and identified for APR to powdery mildew in Liangxing 99.