Zhang Qi, Wang Chenchen, Song Jialu, Gao Liwei, Shen Wenjie, Liu Yi, Chen Daozong, Tan Chen
College of Life Sciences, Gannan Normal University, Ganzhou, China.
Front Plant Sci. 2024 Dec 23;15:1507968. doi: 10.3389/fpls.2024.1507968. eCollection 2024.
Chinese kale ( var. alboglabra), is an annual herb belonging to the Brassica genus of Cruciferae, and is one of the famous specialty vegetables of southern China. Some varieties show bright green leaf (BGL) traits and have better commerciality. However, the genes responsible for this trait remain unidentified.
In this study, gene mapping was measured by BSR-Seq and molecular marker analysis. Gene expression analysis was performed qRT-PCR. Cloning and sequence analysis of candidate genes were also performed.
Genetic analysis revealed that the bright green leaf trait is a dominant trait governed by a single pair of genes. BSR-seq and molecular marker validation mapped the candidate interval to about 1.5 Mb on chromosome C8. After expanding the BC1 population and analyzing recombinant individuals, the interval was refined to approximately 102 kb on chromosome C8 (50,787,908- 50,890,279 bp). There are 24 genes in this region, and after annotation and expression analysis, , associated with wax synthesis, emerged as a key candidate for . We cloned this gene from both parents, revealing significant differences in their promoter regions. A co-segregation primer was subsequently developed and validated in a segregated population, with results consistent with expectations.
The gene is a potential candidate for controlling the bright green leaf trait in Chinese kale, and its function needs to be validated next. Mapping and cloning this gene is crucial to understanding wax synthesis regulation and developing new bright green leafy varieties of Chinese kale.
芥蓝（变种白花芥蓝）是十字花科芸苔属的一年生草本植物，是中国南方著名的特色蔬菜之一。一些品种表现出亮绿叶（BGL）性状，具有更好的商业价值。然而，控制该性状的基因仍未确定。
在本研究中，通过BSR-Seq和分子标记分析进行基因定位。采用qRT-PCR进行基因表达分析。还进行了候选基因的克隆和序列分析。
遗传分析表明，亮绿叶性状是由一对基因控制的显性性状。BSR-seq和分子标记验证将候选区间定位到C8染色体上约1.5 Mb处。在扩大BC1群体并分析重组个体后，该区间被精细定位到C8染色体上约102 kb处（50,787,908 - 50,890,279 bp）。该区域有24个基因，经过注释和表达分析，与蜡质合成相关的基因成为关键候选基因。我们从双亲中克隆了该基因，发现它们的启动子区域存在显著差异。随后开发了一个共分离引物，并在分离群体中进行了验证，结果与预期一致。
该基因是控制芥蓝亮绿叶性状的潜在候选基因，其功能接下来需要验证。定位和克隆该基因对于理解蜡质合成调控以及培育新的亮叶芥蓝品种至关重要。
