Bhat G J, Padmanaban G
Department of Biochemistry, Indian Institute of Science, Bangalore.
Arch Biochem Biophys. 1988 Aug 1;264(2):584-90. doi: 10.1016/0003-9861(88)90324-4.
Inhibitors of heme biosynthesis such as CoCl2, 3-amino-1,2,4-triazole, and thioacetamide block the 3-methylcholanthrene-mediated induction of cytochrome P-450 (c + d) messenger RNAs and their transcription in rat liver. This effect is specific, since the messenger RNA levels for albumin and glutathione transferase (Ya + Yc) and their transcription are not significantly influenced under conditions of heme depletion. Exogenous administration of heme at very low doses (50 micrograms/100 g body wt) is able to completely counteract the effects of the heme biosynthetic inhibitors on cytochrome P-450 (c + d) messenger RNA levels and their transcription. This constitutes a direct proof for the role of heme as a positive regulator of cytochrome P-450 gene transcription.
血红素生物合成抑制剂，如氯化钴、3-氨基-1,2,4-三唑和硫代乙酰胺，可阻断3-甲基胆蒽介导的大鼠肝脏细胞色素P-450（c + d）信使核糖核酸的诱导及其转录。这种作用具有特异性，因为在血红素耗竭的情况下，白蛋白和谷胱甘肽转移酶（Ya + Yc）的信使核糖核酸水平及其转录不受显著影响。以极低剂量（50微克/100克体重）外源性给予血红素能够完全抵消血红素生物合成抑制剂对细胞色素P-450（c + d）信使核糖核酸水平及其转录的影响。这直接证明了血红素作为细胞色素P-450基因转录的正调节因子的作用。
