Univ Lyon, Université Lyon 1, CNRS, Laboratoire de Biométrie et Biologie Evolutive UMR 5558, 69622 Villeurbanne, France; Littoral Environnement et Sociétés (LIENSs), UMR 7266 CNRS - Université de la Rochelle, 2 rue Olympe de Gouges, 17000 La Rochelle, France; INRAE, RiverLy, Ecotoxicology Laboratory, 5 Avenue de la Doua, CS20244, 69625 Villeurbanne Cedex, France.
Littoral Environnement et Sociétés (LIENSs), UMR 7266 CNRS - Université de la Rochelle, 2 rue Olympe de Gouges, 17000 La Rochelle, France.
Environ Int. 2021 Nov;156:106625. doi: 10.1016/j.envint.2021.106625. Epub 2021 May 16.
The use of freshwater invertebrates for biomonitoring has been increasing for several decades, but little is known about relations between external exposure concentration of metals and their biodistribution among different tissues. One and multi-compartments toxicokinetic (TK) models are powerful tools to formalize and predict how a contaminant is bioaccumulated. The aim of this study is to develop modeling approaches to improve knowledge on dynamic of accumulation and fate of Cd and Hg in gammarid's organs. Gammarids were exposed to dissolved metals (11.1 ± 1.2 µg.L of Cd or 0.27 ± 0.13 µg.L of Hg) before a depuration phase. At each sampling days, their organs (caeca, cephalon, intestine and remaining tissues) were separated by dissection before analyses. Results allowed us to determine that i) G.fossarum takes up Cd as efficiently as the mussel M.galloprovincialis, but eliminates it more rapidly, ii) organs which accumulate and depurate the most, in terms of concentrations, are caeca and intestine for both metals; iii) the one-compartment TK models is the most relevant for Hg, while the multi-compartments TK model allows a better fit to Cd data, demonstrating dynamic transfer of Cd among organs.
淡水无脊椎动物在生物监测中的应用已经有几十年的历史了，但人们对金属的外部暴露浓度与其在不同组织中的生物分布之间的关系知之甚少。单室和多室毒代动力学（TK）模型是正式化和预测污染物生物积累方式的有力工具。本研究旨在开发建模方法，以提高对甲壳类动物器官中 Cd 和 Hg 积累和归宿动态的认识。在排泄阶段之前，将食蚊鱼暴露于溶解态金属（11.1±1.2μg·L-1Cd 或 0.27±0.13μg·L-1Hg）中。在每个采样日，通过解剖将它们的器官（盲囊、头节、肠道和剩余组织）分离，然后进行分析。结果表明：i）食蚊鱼对 Cd 的摄取效率与贻贝 M.galloprovincialis 相当，但排泄速度更快；ii）就浓度而言，吸收和排泄最多的器官是盲囊和肠道，这两种金属都是如此；iii）单室 TK 模型最适用于 Hg，而多室 TK 模型则更能拟合 Cd 数据，表明 Cd 在器官之间的动态转移。
