Skilbeck N W, Forsyth W M, Dohnt M
Victorian Department of Agriculture.
Aust Vet J. 1988 Mar;65(3):73-5. doi: 10.1111/j.1751-0813.1988.tb07362.x.
Kidneys from cattle at slaughter were examined for the presence of leptospires. Of 218 (8.3%) kidneys leptospires were isolated from 18; all were identified as Leptospira interrogans serovar hardjo. None of the leptospire-infected kidneys had histopathological lesions indicative of leptospirosis and leptospires were demonstrated in only 2 by immunogold silver staining. Leptospires infected kidneys remained viable for at least 21 days when stored at 4 degrees but became non-viable within 14 days when stored frozen at -15 degrees.
对屠宰牛的肾脏进行检查，以确定是否存在钩端螺旋体。在218个肾脏中（占8.3%），从18个肾脏中分离出钩端螺旋体；所有分离出的钩端螺旋体均被鉴定为问号钩端螺旋体哈焦血清型。没有一个感染钩端螺旋体的肾脏有指示钩端螺旋体病的组织病理学病变，仅通过免疫金银染色在2个肾脏中证实有钩端螺旋体。感染钩端螺旋体的肾脏在4℃储存时至少可存活21天，但在-15℃冷冻储存时14天内就会失去活力。
