Yu Yongbo, Wang Shengcai, Zhang Xuexi, Xu Shuai, Li Yanzhen, Liu Qiaoyin, Yang Yeran, Sun Nian, Liu Yuanhu, Zhang Jie, Guo Yongli, Ni Xin
Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health (NCCH), Beijing, China.
Department of Otolaryngology, Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health (NCCH), Beijing, China.
Transl Pediatr. 2021 Apr;10(4):723-732. doi: 10.21037/tp-20-301.
Thyroid carcinoma is a common pediatric head and neck cancer, of which papillary thyroid cancer (PTC) is the most common type. Previously, we found that thyroid peroxidase () and aldehyde oxidase 1 () were differentially expressed in PTC. This study explored the clinical importance of and in the diagnosis and prognosis of PTC in children.
Both and expression in PTC were analyzed using datasets from Gene Expression Omnibus (GEO). TPO and AOX1 protein levels in plasma from patients with PTC and non-tumor controls were detected via enzyme-linked immunosorbent assay (ELISA). The diagnostic accuracy of TPO and AOX1 was assessed using receiver operating characteristic (ROC) curve analysis. The association between gene expression levels and patient survival was explored using the Kaplan-Meier plotter online database.
The results revealed that and expression was significantly downregulated in four independent datasets (GSE33630, GSE27155, GSE3678, and GSE3467). TPO and AOX1 protein levels in blood plasma were significantly decreased in patients with PTC. Quantitative analysis demonstrated that TPO and AOX1 levels in plasma had satisfactory predictive performance and the ability to discriminate PTC from healthy samples. Prognostic analysis demonstrated that low levels of and were markedly associated with poor survival in patients with PTC.
In summary, these results implied that TPO and AOX1 could serve as novel biomarkers for the diagnosis and prognosis of pediatric PTC.
甲状腺癌是一种常见的儿童头颈癌，其中乳头状甲状腺癌（PTC）是最常见的类型。此前，我们发现甲状腺过氧化物酶（TPO）和醛氧化酶1（AOX1）在PTC中存在差异表达。本研究探讨了TPO和AOX1在儿童PTC诊断和预后中的临床重要性。
使用来自基因表达综合数据库（GEO）的数据集分析PTC中TPO和AOX1的表达。通过酶联免疫吸附测定（ELISA）检测PTC患者和非肿瘤对照者血浆中TPO和AOX1的蛋白水平。使用受试者工作特征（ROC）曲线分析评估TPO和AOX1的诊断准确性。利用在线数据库Kaplan-Meier Plotter探索基因表达水平与患者生存之间的关联。
结果显示，在四个独立数据集（GSE33630、GSE27155、GSE3678和GSE3467）中，TPO和AOX1的表达显著下调。PTC患者血浆中TPO和AOX1的蛋白水平显著降低。定量分析表明，血浆中TPO和AOX1水平具有良好的预测性能，能够区分PTC与健康样本。预后分析表明，TPO和AOX1低水平与PTC患者的不良生存显著相关。
总之，这些结果表明TPO和AOX1可作为儿童PTC诊断和预后的新型生物标志物。
