Centre Armand-Frappier Santé Biotechnologie, Institut national de la recherche scientifique, 531 boul. des Prairies, Laval, QC, H7V 1B7, Canada.
Energy, Mining, and Environment, National Research Council Canada, 6100 Royalmount Ave., Montréal, QC, H4P 2R2, Canada.
FEMS Microbiol Ecol. 2021 Jun 4;97(6). doi: 10.1093/femsec/fiab071.
It is thought that modern wheat genotypes have lost their capacity to associate with soil microbes that would help them acquire nutrients from the soil. To test this hypothesis, ten ancestral and modern wheat genotypes were seeded in a field experiment under low fertilization conditions. The rhizosphere soil was collected, its DNA extracted and submitted to shotgun metagenomic sequencing. In contrast to our hypothesis, there was no significant difference in the global rhizosphere metagenomes of the different genotypes, and this held true when focusing the analyses on specific taxonomic or functional categories of genes. Some genes were significantly more abundant in the rhizosphere of one genotype or another, but they comprised only a small portion of the total genes identified and did not affect the global rhizosphere metagenomes. Our study shows for the first time that the rhizosphere metagenome of wheat is stable across a wide variety of genotypes when growing under nutrient poor conditions.
据认为，现代小麦基因型已经丧失了与土壤微生物结合的能力，而这些微生物可以帮助它们从土壤中获取营养。为了验证这一假说，我们在低施肥条件下进行田间试验，播种了十个祖代和现代小麦基因型。收集根际土壤，提取其 DNA 并进行鸟枪法宏基因组测序。与我们的假设相反，不同基因型的全球根际宏基因组之间没有显著差异，当我们将分析集中在特定的分类或功能基因类别上时，情况也是如此。一些基因在一种基因型的根际中明显更为丰富，但它们仅占所鉴定的总基因的一小部分，并且不会影响全球根际宏基因组。我们的研究首次表明，在养分贫瘠的条件下生长时，小麦的根际宏基因组在各种基因型之间是稳定的。
