Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, India.
Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, West Bengal 721302, India.
Int J Mol Sci. 2020 Nov 24;21(23):8895. doi: 10.3390/ijms21238895.
The plant rhizosphere interfaces an array of microbiomes related to plant growth and development. Cultivar-specific soil microbial communities with respect to their taxonomic structure and specific function have not been investigated explicitly in improving the adaptation of lentil cultivars under rice-fallow ecology. The present study was carried out to decipher the rhizosphere microbiome assembly of two lentil cultivars under rice-fallow ecology for discerning the diversity of microbial communities and for predicting the function of microbiome genes related to nitrogen (N) and phosphorus (P) cycling processes deploying high-throughput whole (meta) genome sequencing. The metagenome profile of two cultivars detected variable microbiome composition with discrete metabolic activity. Cyanobacteria, Bacteroidetes, Proteobacteria, Gemmatimonadetes, and Thaumarchaeota were abundant phyla in the "Farmer-2" rhizosphere, whereas Actinobacteria, Acidobacteria, Firmicutes, Planctomycetes, Chloroflexi, and some incompletely described procaryotes of the "Candidatus" category were found to be robustly enriched the rhizosphere of "Moitree". Functional prediction profiles of the microbial metagenomes between two cultivars revealed mostly house keeping genes with general metabolism. Additionally, the rhizosphere of "Moitree" had a high abundance of genes related to denitrification processes. Significant difference was observed regarding P cycling genes between the cultivars. "Moitree" with a profuse root system exhibited better N fixation and translocation ability due to a good "foraging strategy" for improving acquisition of native P under the nutrient depleted rice-fallow ecology. However, "Farmer-2" revealed a better "mining strategy" for enhancing P solubilization and further transportation to sinks. This study warrants comprehensive research for explaining the role of microbiome diversity and cultivar-microbe interactions towards stimulating microbiome-derived soil reactions regarding nutrient availability under rice-fallow ecology.
植物根际与一系列与植物生长和发育相关的微生物组相互作用。在提高绿豆品种适应水稻-休耕生态的过程中，尚未明确研究品种特异性土壤微生物群落的分类结构和特定功能。本研究旨在阐明两种绿豆品种在水稻-休耕生态下的根际微生物组组装，以辨别微生物群落的多样性，并预测与氮（N）和磷（P）循环过程相关的微生物组基因的功能，方法是利用高通量全（宏）基因组测序。两种品种的宏基因组图谱检测到可变的微生物组组成和离散的代谢活性。蓝细菌、拟杆菌门、变形菌门、芽单胞菌门和泉古菌门是“Farmer-2”根际中丰富的门，而放线菌门、酸杆菌门、厚壁菌门、浮霉菌门、绿弯菌门和一些未完全描述的“候选”原核生物在“Moitree”的根际中被发现大量富集。两种品种的微生物宏基因组之间的功能预测图谱显示，大多数是具有一般代谢的看家基因。此外，“Moitree”的根际中与反硝化过程相关的基因丰度较高。在品种之间观察到 P 循环基因存在显著差异。由于在养分匮乏的水稻-休耕生态下具有良好的“觅食策略”，因此根系茂盛的“Moitree”表现出更好的固氮和转运能力。“Farmer-2”则表现出更好的“开采策略”，以提高对原生 P 的溶解和进一步运输到汇。本研究需要进行全面研究，以解释微生物组多样性和品种-微生物相互作用的作用，激发微生物组衍生的土壤反应，以提高水稻-休耕生态下的养分可用性。
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