The bonding nature of the chemical interaction between trypsin and chitosan based carriers in immobilization process depend on entrapped method: A review.

The review article is dedicated to a comprehensive study of the chemical bond formed during the immobilization of the proteolytic enzyme pancreatic trypsin in chitosan-based polymer matrixes and its derivatives. The main focus of the study is to describe the chemical bond that causes immobilization between chitosan based carriers and trypsin. Because the nature of the chemical bond between the carrier and trypsin is a key factor in determining the area of application of the conjugate. It has been found out that after the chemical nature of functional groups, their degree of ionization, the structure of the chemical cross-linking, the medium pH and ionic strength of chitosan are modified, the mechanism of trypsin immobilization is affected. As a result, the attraction enzyme to the matrix occurs due to polar covalent and hydrogen bonds, as well as electrostatic, hydrophobic, Van der Waals forces. The collected research works on the immobilization of trypsin on chitosan-based carriers have been systematized in the paper and shown schematically in subsystems according to the type of chemical interaction. It has been shown that the immobilization of trypsin on chitosan based matrixes occur more often due to the covalent and hydrogen bonds between the protein and the carrier.