游泳运动对骨关节炎软骨细胞凋亡基因caspase-3表达的影响
Influence of swimming exercise on the expression of apoptotic gene caspase-3 in chondrocytes in osteoarthritis.
作者信息
Zhang Jiting, Qi Baochang, Liu Mingxi, Zhao Tianhao, Tan Lei
机构信息
Department of Traumatic Orthopedics, The First Hospital of Jilin University Changchun, Jilin, China.
出版信息
Am J Transl Res. 2021 Apr 15;13(4):2511-2517. eCollection 2021.
OBJECTIVE
To study swimming exercise's effect on caspase-3 expression in chondrocytes in osteoarthritis (OA).
METHODS
36 SD rats were randomly separated into normal group (n = 12), OA model group (n = 12) and swimming exercise group (n = 12). After modeling, rats received no intervention in model group and swimming exercise once a day (15 min/time) for 4 consecutive weeks in swimming exercise group. After intervention for 4 weeks, specimens were taken to analyze tissue morphology by H&E staining, caspase-3 expression by Western blot and qPCR, chondrocytes apoptosis by TUNEL assay.
RESULTS
HE staining revealed abnormal bone tissue morphology in model group and swimming exercise group with improved morphology after swimming exercise. Model group and swimming exercise group all showed significantly higher Caspase-3 protein level than normal group with lower level after swimming exercise (P < 0.05). Consistently, qPCR showed similar expression profile of caspase-3 mRNA level to the protein level.
CONCLUSION
Swimming exercise can inhibit caspase-3 level and chondrocytes apoptosis in OA, thus improving the joint morphology.
目的
研究游泳运动对骨关节炎（OA）软骨细胞中半胱天冬酶 - 3表达的影响。
方法
将36只SD大鼠随机分为正常组（n = 12）、OA模型组（n = 12）和游泳运动组（n = 12）。建模后，模型组大鼠不接受干预，游泳运动组大鼠连续4周每天进行一次游泳运动（15分钟/次）。干预4周后，取标本通过苏木精 - 伊红（H&E）染色分析组织形态，通过蛋白质印迹法和定量聚合酶链反应（qPCR）检测半胱天冬酶 - 3表达，通过末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测软骨细胞凋亡。
结果
苏木精 - 伊红染色显示模型组和游泳运动组骨组织形态异常，游泳运动后形态有所改善。模型组和游泳运动组半胱天冬酶 - 3蛋白水平均显著高于正常组，游泳运动后水平降低（P < 0.05）。同样，定量聚合酶链反应显示半胱天冬酶 - 3信使核糖核酸水平的表达谱与蛋白水平相似。
结论
游泳运动可抑制骨关节炎中半胱天冬酶 - 3水平和软骨细胞凋亡，从而改善关节形态。
Zotero 插件