Lee Jivianne T, Basak Saroj
Department of Head and Neck Surgery, University of California Los Angeles David Geffen School of Medicine, Los Angeles, California, USA.
Greater Los Angeles Veterans Administration Healthcare System, Los Angeles, California, USA.
OTO Open. 2021 May 4;5(2):2473974X211009232. doi: 10.1177/2473974X211009232. eCollection 2021 Apr-Jun.
Although the etiology of chronic rhinosinusitis remains unknown, environmental factors including airborne pollutants and toxicants are postulated to contribute to its pathogenesis. However, the precise pathomechanisms with which environmental toxicants may contribute to chronic rhinosinusitis are not fully understood. The purpose of this pilot study is to examine the cytotoxic effects of -diethyl--toluamide (DEET), a commonly used pesticide, on sinonasal epithelial cells (SNECs). Sinus mucosa was obtained from 3 subjects without a history of chronic rhinosinusitis. Cultured SNECs were exposed to various concentrations of DEET (0-5 mM) for 6 days. Cell viability, proliferation, and morphologic changes were assessed using the MTT colorimetric dye assay and the Incucyte Live Cell Monitoring System. Statistically significant dose-dependent reduction in cell viability and proliferation was observed between exposure and control groups ( < .05) at all concentrations tested. Dose-dependent cellular morphological changes were also seen. These findings indicate that DEET exposure induces dose-dependent cytotoxicity in sinonasal epithelia.
尽管慢性鼻-鼻窦炎的病因尚不清楚，但包括空气传播污染物和有毒物质在内的环境因素被认为与其发病机制有关。然而，环境有毒物质导致慢性鼻-鼻窦炎的确切病理机制尚未完全明确。本初步研究的目的是检测常用杀虫剂避蚊胺（DEET）对鼻窦上皮细胞（SNECs）的细胞毒性作用。从3名无慢性鼻-鼻窦炎病史的受试者获取鼻窦黏膜。将培养的SNECs暴露于不同浓度的DEET（0 - 5 mM）中6天。使用MTT比色染料法和Incucyte活细胞监测系统评估细胞活力、增殖和形态学变化。在所有测试浓度下，暴露组和对照组之间均观察到细胞活力和增殖呈统计学显著的剂量依赖性降低（P < 0.05）。还观察到剂量依赖性的细胞形态学变化。这些发现表明，接触DEET可诱导鼻窦上皮细胞产生剂量依赖性细胞毒性。
