Kamrad Stephan, Rodríguez-López María, Dey Shoumit, Hoti Mimoza, Wallace Henry, Ralser Markus, Bähler Jürg
University College London, Institute of Healthy Ageing and Department of Genetics, Evolution & Environment, London, U.K.
The Francis Crick Institute, Molecular Biology of Metabolism Laboratory, London, U.K.
MicroPubl Biol. 2021 May 17;2021. doi: 10.17912/micropub.biology.000390.
During meiosis, tethering of parental mitochondria to opposite cell poles inhibits the mixing of mitochondria with different genomes and ensures uniparental inheritance in thestandard laboratory strain of fission yeast. We here investigate mitochondrial inheritance in crosses between natural isolates using tetrad dissection and next-generation sequencing. We find that colonies grown from single spores can sometimes carry a mix of mitochondrial genotypes, that mitochondrial genomes can recombine during meiosis, that in some cases tetrads do not follow the 2:2 segregation pattern, and that certain crosses may feature a weak bias towards one of the parents. Together, these findings paint a more nuanced picture of mitochondrial inheritance in the wild.
在减数分裂过程中，亲代线粒体与细胞两极的连接会抑制不同基因组的线粒体混合，并确保在裂殖酵母的标准实验室菌株中实现单亲遗传。我们在此利用四分体剖析和下一代测序技术研究自然分离株之间杂交的线粒体遗传情况。我们发现，由单个孢子生长而来的菌落有时会携带线粒体基因型的混合体，线粒体基因组在减数分裂过程中会发生重组，在某些情况下，四分体并不遵循2:2的分离模式，并且某些杂交可能会表现出对其中一个亲本的微弱偏向性。这些发现共同描绘了野生环境中线粒体遗传更为细致入微的图景。
