Liotti F S, Pelliccia C, Pezzetti F
Institute of General Biology, University of Perugia, Italy.
Cancer Lett. 1988 Aug 15;41(2):235-42. doi: 10.1016/0304-3835(88)90121-8.
The effects of treatment with reduced glutathione, cysteine and ascorbic acid on chick embryo fibroblasts and hepatocytes cultured in vitro in the presence of [G-3H]benzo[a]pyrene ([3H]BP) were studied in an attempt to evaluate the capacity of these antioxidants to interfere with the binding of the carcinogen to DNA in these two cell populations. The results revealed that in the absence of treatment with antioxidants, the carcinogen bound to the DNA of the hepatocytes less than it did to that of the fibroblasts, despite the fact that the two cell populations manifest more or less the same uptake of [G-3H]benzo[a]pyrene from the medium. Moreover the hepatocytes, unlike the fibroblasts, seemed to reap little advantage from the known capacity of the antioxidants to interfere with the binding of the carcinogen to DNA.
研究了用还原型谷胱甘肽、半胱氨酸和抗坏血酸处理对在[G-3H]苯并[a]芘（[3H]BP）存在下体外培养的鸡胚成纤维细胞和肝细胞的影响，以评估这些抗氧化剂干扰致癌物与这两种细胞群体中DNA结合的能力。结果显示，在未用抗氧化剂处理的情况下，尽管这两种细胞群体从培养基中摄取[G-3H]苯并[a]芘的量大致相同，但致癌物与肝细胞DNA的结合量少于与成纤维细胞DNA的结合量。此外，与成纤维细胞不同，肝细胞似乎并未从抗氧化剂干扰致癌物与DNA结合的已知能力中获得多少益处。
