Institute of Biochemistry, Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria.
Methods Mol Biol. 2021;2318:87-117. doi: 10.1007/978-1-0716-1476-1_6.
The MYC oncogene was originally identified as a transduced allele (v-myc) in the genome of the highly oncogenic avian retrovirus MC29. The protein product (MYC) of the cellular MYC (c-myc) protooncogene represents the key component of a transcription factor network controlling the expression of a large fraction of all human genes. MYC regulates fundamental cellular processes like growth control, metabolism, proliferation, differentiation, and apoptosis. Mutational deregulation of MYC, leading to increased levels of the MYC protein, is a frequent event in the etiology of human cancers. In this chapter, we describe cell systems and experimental strategies to quantify the oncogenic potential of MYC alleles, to test MYC inhibitors, and to monitor MYC-specific protein-protein interactions that are relevant for the cell transformation process. We also describe experimental procedures to study the evolutionary origin of MYC and to analyze structure, function, and regulation of the ancestral MYC proto-oncogenes.
MYC 癌基因最初是在高度致癌的禽源逆转录病毒 MC29 的基因组中被鉴定为转导等位基因（v-myc）。细胞 MYC（c-myc）原癌基因的蛋白产物（MYC）代表转录因子网络的关键组成部分，该网络控制着人类大多数基因的表达。MYC 调节着基本的细胞过程，如生长控制、代谢、增殖、分化和凋亡。MYC 的突变失调控导致 MYC 蛋白水平升高，这是人类癌症发病机制中的一个常见事件。在本章中，我们描述了细胞系统和实验策略，用于量化 MYC 等位基因的致癌潜力，测试 MYC 抑制剂，并监测与细胞转化过程相关的 MYC 特异性蛋白-蛋白相互作用。我们还描述了研究 MYC 的进化起源以及分析原始 MYC 原癌基因的结构、功能和调节的实验程序。
