Warburton M J, Ferns S A, Hughes C M
Department of Histopathology, St George's Hospital Medical School, London, UK.
Cell Biol Int Rep. 1988 May;12(5):397-405. doi: 10.1016/0309-1651(88)90064-1.
A rat mammary myoepithelial cell line (Rama 401) grown on plastic produces 5 times more collagen (largely type IV) than a mammary epithelial cell line (Rama 704) grown on the same surface. When the cells are grown on collagen gels, the amount of collagen produced by Rama 704 cells increases 3.3 times, whereas there is no increase in collagen production by Rama 401 cells. Increased production of collagen by Rama 704 cells is due to both an increased rate of synthesis and a decreased rate of degradation. These results indicate that for mammary epithelial cells, unlike myoepithelial cells, the rate of production of collagen can be regulated by the extracellular matrix.
在塑料表面生长的大鼠乳腺肌上皮细胞系（Rama 401）产生的胶原蛋白（主要是IV型）比在同一表面生长的乳腺上皮细胞系（Rama 704）多5倍。当细胞在胶原蛋白凝胶上生长时，Rama 704细胞产生的胶原蛋白量增加3.3倍，而Rama 401细胞的胶原蛋白产量没有增加。Rama 704细胞胶原蛋白产量的增加是由于合成速率增加和降解速率降低。这些结果表明，对于乳腺上皮细胞，与肌上皮细胞不同，胶原蛋白的产生速率可由细胞外基质调节。
