College of Science, Health, Engineering and Education, Murdoch University, Murdoch, Western Australia, 6150, Australia; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia, 6076, Australia.
Parasitol Int. 2021 Oct;84:102386. doi: 10.1016/j.parint.2021.102386. Epub 2021 May 18.
An Isospora species, Isospora amphiboluri, originally described by Canon in 1967 and later by McAllister et al. (1995), was isolated from a central netted dragon (Ctenophorus nuchalis) housed at a wildlife rehabilitation centre in Perth, Western Australia. Sporulated oocysts of Isospora amphiboluri (n = 30) are spherical, 24.2 (26.5-23.0) μm in length and 23.9 (22.4-25.9) μm in width, with a shape index of 1.01. The bilayered oocyst wall is smooth and light-yellow in color. Polar granule, oocyst residuum and micropyle are absent. The sporocysts are lemon-shaped, 15.7 (15.2-18.0) × 10.2 (8.9-11.2) μm, with a shape index (length/width) of 1.53. Stieda and substieda bodies are present, the Stieda body being small and hemidome-shaped and the substieda half-moon-shaped. Each sporocyst contains four vermiform sporozoites arranged head to tail. The sporozoites are 11.7 (9.9-16.2) × 3.0 (2.4-3.5) μm, with a shape index (length/width) of 3.87. A sporocyst residuum is present. Sporozoites contain a central nucleus with a finely distributed granular residuum. Comparison of oocyst measurements and their features with other valid Isospora species from hosts in the Agamid family confirmed that this Isospora species is Isospora amphiboluri. Molecular characterization of I. amphiboluri at the 18S rRNA and MTCOI loci showed the highest similarity with I. amphiboluri from the central bearded dragon, 99.8% and 99.7% respectively. This is the first report of I. amphiboluri from a central netted dragon in Australia.
一种等孢球虫，即最初由 Canon 于 1967 年描述，后由 McAllister 等人（1995 年）描述的等孢球虫 amphiboluri，从澳大利亚珀斯的一个野生动物康复中心饲养的中央网纹龙（Ctenophorus nuchalis）中分离出来。等孢球虫 amphiboluri 的孢子化卵囊（n = 30）呈球形，长 24.2（26.5-23.0）μm，宽 23.9（22.4-25.9）μm，形态指数为 1.01。双层卵囊壁光滑，呈浅黄色。无极粒、卵囊残余物和卵盖。孢子囊呈柠檬形，长 15.7（15.2-18.0）×10.2（8.9-11.2）μm，形态指数（长/宽）为 1.53。斯氏和副斯氏体存在，斯氏体小，呈半穹顶形，副斯氏体呈半月形。每个孢子囊含有 4 条排列成头尾相连的蠕虫状的孢子虫。孢子虫长 11.7（9.9-16.2）×3.0（2.4-3.5）μm，形态指数（长/宽）为 3.87。有孢子囊残余物。孢子虫含有一个中央核和一个细粒状的残余物。将卵囊测量值及其特征与从鬣蜥科宿主中分离出的其他有效等孢球虫进行比较，证实该等孢球虫为等孢球虫 amphiboluri。在 18S rRNA 和 MTCOI 基因座上对 I. amphiboluri 的分子特征进行鉴定，结果显示其与来自中央鬃狮蜥的 I. amphiboluri 的相似度最高，分别为 99.8%和 99.7%。这是在澳大利亚首次从中央网纹龙中报告发现 I. amphiboluri。
