Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Japan; Department of Immunology, Faculty of Medicine, University of Tsukuba, Japan.
Department of Immunology, Faculty of Medicine, University of Tsukuba, Japan; R&D Center for Innovative Drug Discovery, University of Tsukuba, Japan.
Biochem Biophys Res Commun. 2021 Jul 5;561:101-105. doi: 10.1016/j.bbrc.2021.04.097. Epub 2021 May 18.
Mast cells (MCs) are present in various organs including the skin, peritoneal cavity, lung, and intestine and involved in the development of allergic diseases and host defense against infection. However, the regulatory mechanism of mast cell activation remains incompletely understood. We found in a database that Clec12b encoding a C-type lectin receptor Clec12b is preferentially expressed in skin MCs in mice. However, neither MCs in other tissues such as trachea, tongue, esophagus, or peritoneal cavity nor most lymphocytes and myeloid cells express Clec12b. To analyze the protein expression of Clec12b, we newly generated a monoclonal antibody (named TX109), which recognizes both mouse and human Clec12b. Consistent with the gene expression profile, flow cytometry analysis demonstrated that Clec12b is expressed only on MCs in the skin, but not on any other immune cell types in various tissues, in mice. Similarly, Clec12b is also expressed on skin MCs, but not on circulating lymphocytes and myeloid cells, in humans. Our results suggest that Clec12b plays an important role in the regulation of MCs activation in the skin.
肥大细胞（MCs）存在于各种器官中，包括皮肤、腹腔、肺和肠道，参与过敏疾病的发展和宿主对感染的防御。然而，肥大细胞激活的调节机制仍不完全清楚。我们在数据库中发现，编码 C 型凝集素受体 Clec12b 的 Clec12b 在小鼠皮肤 MCs 中优先表达。然而，气管、舌头、食管或腹腔等其他组织中的 MCs以及大多数淋巴细胞和髓样细胞均不表达 Clec12b。为了分析 Clec12b 的蛋白表达，我们新生成了一种单克隆抗体（命名为 TX109），它可识别小鼠和人源 Clec12b。与基因表达谱一致，流式细胞术分析表明，Cle c12b 仅在皮肤 MCs 上表达，而在小鼠的各种组织中的任何其他免疫细胞类型上均不表达。同样，Cle c12b 也在人类的皮肤 MCs 上表达，但不在循环淋巴细胞和髓样细胞上表达。我们的结果表明，Cle c12b 在皮肤 MCs 激活的调节中发挥重要作用。
