School of Life Sciences, Taizhou University, Jiaojiang, 318000, PR China.
School of Life Sciences, Taizhou University, Jiaojiang, 318000, PR China.
Chemosphere. 2021 Oct;281:130418. doi: 10.1016/j.chemosphere.2021.130418. Epub 2021 Apr 26.
The study aims to identify relations of denitrifying bacterial and fungal communities to nitrogen removals in vertical flow wetland microcosms (VFWMs) using four macrophyte species (Iris pseudacorus, Canna glauca, Scirpus validus and Cyperus alternifolius) and three species richness levels (unplanted, monocultured and 4-species mixture) as fixed factors. Results showed that among four macrophyte species, only Canna glauca planting significantly decreased nitrate removal by 87.7% in the VFWMs. The 4-species mixture improved TN and nitrate removals by 84.0% and 91.3%, but decreased ammonium removal by 94.5%. Heatmap and nonmetric multidimensional scaling analyses identified a significant difference in denitrifying bacterial community structure across macrophyte richness levels, but did not identify the difference in denitrifying fungal communities. The redundancy analysis revealed that denitrifying bacterial community individually explained 99.4% and 93.0% variance of nitrogen removals among four macrophyte species and across macrophyte richness levels, while the fungal community only explained 30.7% and 21.8% variance of nitrogen removals. Overall, the macrophyte richness and bacterial denitrifiers are the critical factors of nitrogen removals in the VFWMs, thus providing useful data to design a vertical flow constructed wetland at a full scale.
本研究旨在利用四种植物物种（鸢尾、美人蕉、香蒲和水葱）和三种物种丰富度水平（无植物、单一种植和 4 种混合种植）作为固定因素，确定垂直流湿地微系统（VFWMs）中脱氮细菌和真菌群落与氮去除之间的关系。结果表明，在四种植物物种中，只有美人蕉种植显著降低了 VFWMs 中的硝酸盐去除率，达 87.7%。4 种混合种植提高了 TN 和硝酸盐的去除率，分别为 84.0%和 91.3%，但降低了铵盐的去除率 94.5%。热图和非度量多维尺度分析确定了脱氮细菌群落结构在不同植物丰富度水平之间存在显著差异，但未确定脱氮真菌群落的差异。冗余分析表明，脱氮细菌群落分别解释了四种植物物种和不同植物丰富度水平之间氮去除率的 99.4%和 93.0%的方差，而真菌群落仅解释了氮去除率的 30.7%和 21.8%的方差。总体而言，植物丰富度和细菌脱氮菌是 VFWMs 中氮去除的关键因素，因此为设计全规模垂直流人工湿地提供了有用的数据。
