Bützow R, Alfthan H, Stenman U H, Suikkari A M, Bohn H, Seppälä M
First Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.
Clin Chem. 1988 Aug;34(8):1591-3.
This time-resolved immunofluorometric assay (IFMA) developed for measurement of placental protein 5 (PP5) involves two antibodies: a monoclonal anti-PP5 antibody attached to a solid phase and an europium(III) chelate-labeled polyclonal anti-PP5 antibody as a tracer. The measuring range is 0.05-100 micrograms/L and the detection limit is 20 times lower than that of a PP5 radioimmunoassay (RIA) performed with the same polyclonal antiserum. By IFMA, PP5 could be detected and quantified in all plasma and serum samples of nonpregnant and pregnant individuals, whereas PP5 was undetectable by RIA in serum of healthy men and nonpregnant women. The mean concentration of PP5 in sera from men was 0.43 micrograms/L (SD 0.13, range 0.19-0.75, n = 47) and in sera from nonpregnant women 0.49 micrograms/L (SD 0.19, range 0.20-0.90, n = 41). PP5 concentrations in serum showed no systematic variation during the menstrual cycle. In serum samples from 60 pregnant women the results obtained by IFMA and RIA correlated well (r = 0.97).
这种为检测胎盘蛋白5（PP5）而开发的时间分辨免疫荧光测定法（IFMA）涉及两种抗体：一种附着于固相的抗PP5单克隆抗体和一种作为示踪剂的铕（III）螯合物标记的抗PP5多克隆抗体。测量范围为0.05 - 100微克/升，检测限比使用相同多克隆抗血清进行的PP5放射免疫测定法（RIA）低20倍。通过IFMA，可以在非孕妇和孕妇的所有血浆和血清样本中检测和定量PP5，而在健康男性和非孕妇血清中，RIA检测不到PP5。男性血清中PP5的平均浓度为0.43微克/升（标准差0.13，范围0.19 - 0.75，n = 47），非孕妇血清中为0.49微克/升（标准差0.19，范围0.20 - 0.90，n = 41）。血清中PP5浓度在月经周期中无系统性变化。在60名孕妇的血清样本中，IFMA和RIA所得结果相关性良好（r = 0.97）。
