Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
School of Life Sciences, Center for Bioinformatics, Center for Statistical Science, Peking University, Beijing, China.
Nat Commun. 2021 May 21;12(1):3017. doi: 10.1038/s41467-021-23320-8.
Extended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno-free culture system for culturing and deriving human EPS cells in vitro. Xeno-free human EPS cells can be long-term and genetically stably maintained in vitro, as well as preserve their embryonic and extraembryonic developmental potentials. Furthermore, the xeno-free culturing system also permits efficient derivation of human EPS cells from human fibroblast through reprogramming. Our study could have broad utility in future applications of human EPS cells in biomedicine.
扩展多能干细胞（EPS 细胞）在生成合成胚胎、定向分化和疾病建模方面显示出巨大的应用潜力。然而，缺乏无动物培养条件极大地限制了它们的应用。在这里，我们报告了一种化学定义和无动物的培养系统，用于体外培养和衍生人类 EPS 细胞。无动物的人类 EPS 细胞可以在体外长期遗传稳定地维持，并且保持其胚胎和胚胎外发育潜能。此外，无动物培养系统还允许通过重编程从人成纤维细胞中高效地获得人类 EPS 细胞。我们的研究在未来人类 EPS 细胞在生物医学中的应用中具有广泛的用途。
