Department of Chemistry, The University of Burdwan, Burdwan, West Bengal, India.
Departamento de Microbiologia, CCB, Universidade Estadual de Londrina, Caixa Postal 6001, 86057-970 Londrina, PR, Brazil.
Int J Biol Macromol. 2021 Jul 31;183:1419-1426. doi: 10.1016/j.ijbiomac.2021.05.107. Epub 2021 May 20.
Herpes simplex virus type 1(HSV-1) attaches to cell surface heparan sulfate aiming to enter into susceptible cells. In this work, we utilized a sulfur trioxide-pyridine in N,N-dimethylformamide (SO·Pyr/DMF) based amalgamated extraction-sulfation procedure for producing arabinogalactan sulfates from Anogeissus latifolia gum. Chemical, chromatographic, spectroscopic and chemical data revealed that the derived polymers contained varying molecular masses (31-69 kDa) and degrees of sulfation (0.1-0.5), but similar saccharide compositions. The highly active polymer (HSV-1: IC and SI, respectively, of 127 μg/mL and 15.7) was a 69 kDa arabinogalactan holding sulfates at O-5 of arabinofuranosyl residues and showed no cytotoxicity as far as 2 mg/mL concentration. This chemically sulfated macromolecule acted by obstructing viral attachment and entry. Thus, SO·Pyr/DMF is suitable for producing new molecules with varied structures and altered pharmacological activities from plant sources.
单纯疱疹病毒 1 型 (HSV-1) 附着在细胞表面的硫酸乙酰肝素上，以进入易感细胞。在这项工作中，我们利用三氧化硫-吡啶在 N，N-二甲基甲酰胺（SO·Pyr/DMF）中的汞齐萃取-磺化方法，从印楝胶中提取阿拉伯半乳聚糖硫酸盐。化学、色谱、光谱和化学数据表明，衍生的聚合物具有不同的分子量（31-69 kDa）和硫酸化程度（0.1-0.5），但具有相似的糖组成。高活性聚合物（HSV-1：IC 和 SI 分别为 127 μg/mL 和 15.7）是一种 69 kDa 的阿拉伯半乳聚糖，在阿拉伯呋喃糖残基的 O-5 位上带有硫酸盐，在 2 mg/mL 浓度下没有细胞毒性。这种化学磺化的大分子通过阻止病毒附着和进入来发挥作用。因此，SO·Pyr/DMF 适合从植物来源生产具有不同结构和改变的药理学活性的新分子。
