鉴定蝎毒来源的抗菌肽Hp1404作为一种抗碳青霉烯类耐药鲍曼不动杆菌的新型抗菌剂。
Identification of the scorpion venom-derived antimicrobial peptide Hp1404 as a new antimicrobial agent against carbapenem-resistant Acinetobacter baumannii.
作者信息
Luo Xudong, Ye Xiangdong, Ding Li, Zhu Wen, Zhao Zhiwen, Luo Dan, Liu Na, Sun Luyue, Chen Zongyun
机构信息
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, China; Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan, 442000, China.
Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, China; Department of Clinical Laboratory, Dongfeng Hospital, Hubei University of Medicine, Shiyan, 442000, China.
出版信息
Microb Pathog. 2021 Aug;157:104960. doi: 10.1016/j.micpath.2021.104960. Epub 2021 May 20.
Carbapenem-resistant Acinetobacter baumannii (CRAB) is becoming a troublesome issue worldwide, and anti-CRAB drug research and development is urgently needed. To identify new anti-CRAB drug leads, we investigated seven scorpion venom-derived α-helical peptides that differ in their sequence composition and length. Three peptides, Hp1404, ctriporin and Im5, showed antimicrobial activities against Acinetobacter baumannii. Further antimicrobial assays revealed that Hp1404 exhibited the best cell selectivity with high anti-CRAB and low hemolytic activities. Fluorescence assays demonstrated that Hp1404 can induce dose-dependent disruptions of the bacterial cell membrane, implying a membrane-lytic mode of action. Taken together, our work sheds light on the potential of the scorpion venom-derived peptide Hp1404 for the development of novel antimicrobial agents against CRAB infections.
耐碳青霉烯类鲍曼不动杆菌（CRAB）正在成为一个全球性的棘手问题，因此迫切需要开展抗CRAB药物的研发工作。为了鉴定新的抗CRAB药物先导物，我们研究了七种源自蝎毒的α-螺旋肽，它们在序列组成和长度上存在差异。三种肽，即Hp1404、ctriporin和Im5，对鲍曼不动杆菌显示出抗菌活性。进一步的抗菌试验表明，Hp1404表现出最佳的细胞选择性，具有高抗CRAB活性和低溶血活性。荧光试验证明，Hp1404可诱导细菌细胞膜发生剂量依赖性破坏，这意味着其作用方式为膜溶解。综上所述，我们的工作揭示了源自蝎毒的肽Hp1404在开发抗CRAB感染新型抗菌剂方面的潜力。
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