Department of Veterinary Medicine, Università Degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy.
Department of Veterinary Medicine, Università Degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy.
Vet Immunol Immunopathol. 2021 Jul;237:110269. doi: 10.1016/j.vetimm.2021.110269. Epub 2021 May 15.
Pectin is a dietary fibre composed of galacturonic acid, primarily found in the citrus fruits' cell walls. Citrus pectin (CP) has demonstrated antioxidative, anticancer, and anti-inflammatory properties in humans and animals. In broilers, CP supplementation improves energy utilization and nutrient digestibility, but limited information on its effects on chicken immunity is available so far. This study aimed to assess the in vitro impact of CP on chicken monocytes' immune response. Cells were purified from whole blood of healthy chickens and incubated with increasing concentrations (0, 0.25, 0.5, 0.75, 1 mg/mL) of CP to determine CP working concentration. The effects of different CP concentrations on cells' apoptosis and viability were assessed by measuring caspase-3 and -7 and the cells' metabolic activity (MTT assay), respectively. CP had no dose-dependent effect on monocyte apoptosis and viability.Then, the effects of CP (0.5 mg/mL) on chicken monocytes' chemotaxis and phagocytosis were assessed by measuring transwell migration and fluorescein-labelled E. coli incorporation, respectively. CP inhibited both monocytes' chemotaxis and phagocytosis.These data demonstrate that CP exerts an immunomodulatory role in chicken monocytes, supporting its integration in nutrition strategies that might be beneficial for the animal's immunity and health.
果胶是一种由半乳糖醛酸组成的膳食纤维，主要存在于柑橘类水果的细胞壁中。柑橘果胶（CP）已被证明在人类和动物中具有抗氧化、抗癌和抗炎特性。在肉鸡中，CP 补充剂可提高能量利用率和营养物质消化率，但迄今为止，关于其对鸡免疫力影响的信息有限。本研究旨在评估 CP 对鸡单核细胞免疫反应的体外影响。细胞从健康鸡的全血中分离出来，并与不同浓度（0、0.25、0.5、0.75、1mg/ml）的 CP 孵育，以确定 CP 的工作浓度。通过测量 caspase-3 和 -7 分别评估不同 CP 浓度对细胞凋亡和活力的影响，以及通过 MTT 测定法评估细胞代谢活性。CP 对单核细胞凋亡和活力没有剂量依赖性影响。然后，通过测量 Transwell 迁移和荧光标记的大肠杆菌掺入，分别评估 CP（0.5mg/ml）对鸡单核细胞趋化性和吞噬作用的影响。CP 抑制单核细胞的趋化性和吞噬作用。这些数据表明 CP 对鸡单核细胞发挥免疫调节作用，支持其整合到营养策略中，这可能对动物的免疫力和健康有益。
