Li Mingyuan, Wang Jilian, Yao Tuo, Zhang Tian, Zhou Qian
College of Grassland Science, Gansu Agricultural University, Lanzhou, 730000, China.
College of Biologic and Geographic Sciences, Kashi University, Kashi, 844000, China.
Curr Microbiol. 2021 Jul;78(7):2720-2732. doi: 10.1007/s00284-021-02536-3. Epub 2021 May 23.
The study of the rhizosphere microbial community in salinized soils aids in the elucidation of new and important microbial functional groups, which is of great importance in vegetation restoration and ecological reconstruction of salinized soil. The rhizosphere soil bacterial diversity and community structures of four halophytes, including Kalidium foliatum, Lycium ruthenicum, Karelinia caspia and Phragmites australis, typically distributed in the saline-alkaline land of Southern Xinjiang, China, were studied using an Illumina paired-end sequence platform. The study aims to reveal the alpha diversity, species composition, abundance and the differences of rhizosphere bacteria among the four halophytes, explore their correlation with environmental factors. The results showed that the highest bacterial species diversity was associated with P. communis, followed by K. foliatum, K. caspia, and L. ruthenicum. The species richness was the lowest for L. ruthenicum, while the others showed no significant difference. Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes were the most dominant phyla. And Bacillus and Halomonas were the most common dominant genera. The bacterial communities associated with K. foliatum and K. caspia were similar, while that of L. ruthenicum was significantly different from other halophytes. Soil total nitrogen and total phosphorus, soil organic matter, soil water content, electronic conductivity and pH were identified as the key factors affecting bacterial abundance associated with the assayed halophytes. These results indicate that the four halophytes evaluated in the present study have a similar rhizosphere bacterial community structure due to their being in the same region. However, the bacterial abundance is different among the plant species, and soil properties are the important factors driving the structures of bacterial communities.
对盐渍化土壤根际微生物群落的研究有助于阐明新的重要微生物功能群，这对盐渍化土壤的植被恢复和生态重建具有重要意义。利用Illumina双端测序平台，研究了中国新疆南部盐碱地典型分布的四种盐生植物，包括盐爪爪、黑果枸杞、里海盐角草和芦苇的根际土壤细菌多样性和群落结构。本研究旨在揭示这四种盐生植物根际细菌的α多样性、物种组成、丰度及差异，探讨它们与环境因子的相关性。结果表明，细菌物种多样性最高的是芦苇，其次是盐爪爪、里海盐角草和黑果枸杞。黑果枸杞的物种丰富度最低，而其他植物之间没有显著差异。厚壁菌门、变形菌门、放线菌门和拟杆菌门是最主要的菌门。芽孢杆菌属和嗜盐单胞菌属是最常见的优势属。盐爪爪和里海盐角草相关的细菌群落相似，而黑果枸杞的细菌群落与其他盐生植物显著不同。土壤全氮、全磷、土壤有机质、土壤含水量、电导率和pH值被确定为影响所测盐生植物相关细菌丰度的关键因素。这些结果表明，本研究中评估的四种盐生植物由于处于同一区域，具有相似的根际细菌群落结构。然而，不同植物物种间细菌丰度不同，土壤性质是驱动细菌群落结构的重要因素。
