Division of Paediatrics, University of Western Australia, Western Australia, Australia; Wesfarmers Centre of Vaccines and Infectious Diseases, Telethon Kids Institute, Western Australia, Australia.
Papua New Guinea Institute of Medical Research, Goroka, Eastern Highlands Province, Papua New Guinea.
Vaccine. 2021 Jun 11;39(26):3486-3492. doi: 10.1016/j.vaccine.2021.05.022. Epub 2021 May 21.
Nasopharyngeal colonisation with nontypeable Haemophilus influenzae (NTHi) is associated with development of infections including pneumonia and otitis media. The 10-valent pneumococcal conjugate vaccine (PCV10) uses NTHi Protein D (PD) as a carrier. Papua New Guinean children have exceptionally early and dense NTHi carriage, and high rates of NTHi-associated disease. Vaccination with PCV10 could potentially reduce NTHi carriage and disease in this population by inducing a NTHi PD immune response.
Serum and nasopharyngeal swabs were collected from 101 Papua New Guinean children at 1, 4, 9, 10, 23 and 24 months of age. Children received PCV10 (n = 55) or PCV13 (not containing NTHi PD) (n = 46) at 1, 2 and 3 months of age. NTHi carriage density was measured in swabs by qPCR. Serum PD-IgG levels were measured by bead-based immunoassay.
Papua New Guinean children did naturally develop PD-IgG antibodies whose levels were increased at 4 months of age with PCV10 vaccination at 1-2-3 months. Despite this, most children were colonised with NTHi by 4 months of age (~95%) regardless of being vaccinated with PCV10 or PCV13, and PCV10 had no impact on NTHi carriage density.
Early vaccination of infants with PCV10 elicited a robust PD antibody response but this had no impact on NTHi carriage.
ClinicalTrials.gov CTN NCT01619462.
鼻咽部定植的流感嗜血杆菌（NTHi）与肺炎和中耳炎等感染的发展有关。10 价肺炎球菌结合疫苗（PCV10）使用 NTHi 蛋白 D（PD）作为载体。巴布亚新几内亚的儿童鼻咽部定植 NTHi 的时间异常早且密集，NTHi 相关疾病的发生率也很高。接种 PCV10 可能通过诱导 NTHi PD 免疫应答，减少该人群中 NTHi 的定植和疾病。
101 名巴布亚新几内亚儿童在 1、4、9、10、23 和 24 个月时采集血清和鼻咽拭子。儿童在 1、2 和 3 个月时接受 PCV10（n=55）或不包含 NTHi PD 的 PCV13（n=46）接种。qPCR 检测拭子中 NTHi 的定植密度。通过基于珠子的免疫测定法测量血清 PD-IgG 水平。
巴布亚新几内亚儿童自然产生 PD-IgG 抗体，其水平在 4 个月时接种 PCV10 后增加。尽管如此，大多数儿童在 4 个月时已被 NTHi 定植（~95%），无论接种 PCV10 还是 PCV13，PCV10 对 NTHi 的定植密度均无影响。
婴儿早期接种 PCV10 可引起强烈的 PD 抗体反应，但对 NTHi 的定植无影响。
ClinicalTrials.gov CTN NCT01619462。
