Tohamy Adel F, Hussein Shaymaa, Moussa Ihab M, Rizk Hamdy, Daghash Samer, Alsubki Roua A, Mubarak Ayman S, Alshammari Hanan O, Al-Maary Khalid S, Hemeg Hassan A
Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Egypt.
Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Saudi J Biol Sci. 2021 May;28(5):2755-2761. doi: 10.1016/j.sjbs.2021.03.039. Epub 2021 Mar 18.
Cyclophosphamide is anticancer drug with a well-Known nephrotoxicity. This work was applied to study the lucrative antioxidant influence of metformin as co-therapy on the nephrotoxicity induced by cyclophosphamide in the treatment of different cancer diseases. Four groups of male Sprague Dawley rats were used; Control group (C) received single I.P. injection of 0.2 ml saline, Metformin (MET) group received daily gavage of 200 mg/kg metformin for two weeks, Cyclophosphamide (CP) group received single I.P. injection of 200 mg/kg CP, Protector group (CP.MET) received daily gavage of 200 mg/kg metformin for two weeks and single I.P. injection of 200 mg/kg CP at day 7. By day 14 rats were euthanized. Samples were collected from kidney tissues and blood for kidney function evaluation, histopathological and assessment of oxidative stress markers. The results disclosed that CP yields many functional and structural damage to the kidney, worsened oxidative stress markers and kidney function indicators. The protector group displayed better kidney tissue morphology, acceptable kidney function indicators as well as satisfactory oxidative stress markers. In assumption, metformin could be combined with CP owing to its lucrative effect counter to CP persuaded nephrotoxicity.
环磷酰胺是一种具有众所周知肾毒性的抗癌药物。这项工作旨在研究二甲双胍作为联合治疗药物对环磷酰胺在治疗不同癌症疾病时所诱导的肾毒性的有益抗氧化作用。使用了四组雄性斯普拉格-道利大鼠；对照组（C）腹腔注射0.2毫升生理盐水，二甲双胍（MET）组连续两周每日灌胃200毫克/千克二甲双胍，环磷酰胺（CP）组腹腔注射200毫克/千克环磷酰胺，保护组（CP.MET）连续两周每日灌胃200毫克/千克二甲双胍，并在第7天腹腔注射200毫克/千克环磷酰胺。到第14天对大鼠实施安乐死。从肾脏组织和血液中采集样本，用于评估肾功能、进行组织病理学检查以及评估氧化应激标志物。结果显示，环磷酰胺对肾脏造成了许多功能和结构损伤，使氧化应激标志物和肾功能指标恶化。保护组显示出较好的肾脏组织形态、可接受的肾功能指标以及令人满意的氧化应激标志物。据此推测，由于二甲双胍对环磷酰胺所致肾毒性具有有益作用，它可以与环磷酰胺联合使用。
