血小板活化因子降低培养的人成纤维细胞中低密度脂蛋白的降解并改变脂质代谢。

PAF-acether decreases low density lipoprotein degradation and alters lipid metabolism in cultured human fibroblasts.

作者信息

Mazière J C, Mazière C, Auclair M, Mora L, Polonovski J

机构信息

Faculté de Médecine Saint-Antoine, CNRS UA 524, Paris, France.

出版信息

FEBS Lett. 1988 Aug 15;236(1):115-8. doi: 10.1016/0014-5793(88)80296-5.

DOI:10.1016/0014-5793(88)80296-5

PMID:3402608

Abstract

A 24 h pretreatment of human cultured fibroblasts with PAF-acether (PAF) induced a decrease in LDL degradation and a correlative accumulation of undegraded LDL. LDL binding was not significantly affected. Sterol and triacylglycerol synthesis from sodium acetate was enhanced whereas phospholipid synthesis decreased. Oleic acid incorporation into cholesteryl ester was markedly inhibited, whereas incorporation into triacylglycerols was increased. A decrease in the percentage of phosphatidylcholine and an increase in the percentage of phosphatidylethanolamine were found using sodium [32P]orthophosphate as precursor. These effects of PAF on LDL and lipid metabolism could be related to perturbations in membrane structure characteristics, leading to a delay in LDL delivery to lysosomes, and to modification of the activity of some key enzymes of lipid metabolism.

摘要

用血小板活化因子（PAF）对人培养成纤维细胞进行24小时预处理，可导致低密度脂蛋白（LDL）降解减少以及未降解LDL的相关积累。LDL结合未受到显著影响。乙酸钠合成固醇和三酰甘油增强，而磷脂合成减少。油酸掺入胆固醇酯受到明显抑制，而掺入三酰甘油增加。以前体[32P]正磷酸钠发现磷脂酰胆碱百分比降低，磷脂酰乙醇胺百分比增加。PAF对LDL和脂质代谢的这些影响可能与膜结构特征的扰动有关，导致LDL输送到溶酶体的延迟，以及脂质代谢一些关键酶活性的改变。