Shafaei Armaghan, Rees Joanna, Christophersen Claus T, Devine Amanda, Broadhurst David, Boyce Mary C
Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, Joondalup, WA, 6027, Australia.
School of Medical and Health Sciences, Edith Cowan University, Joondalup, WA, 6027, Australia.
Data Brief. 2021 Apr 22;36:107091. doi: 10.1016/j.dib.2021.107091. eCollection 2021 Jun.
Measuring bile acids in feces has an important role in disease prevention, diagnosis, treatment, and can be considered a measure of health status. Therefore, the primary aim was to develop a sensitive, robust, and high throughput liquid chromatography tandem mass spectrometry method with minimal sample preparation for quantitative determination of bile acids in human feces applicable to large cohorts. Due to the chemical diversity of bile acids, their wide concentration range in feces, and the complexity of feces itself, developing a sensitive and selective analytical method for bile acids is challenging. A simple extraction method using methanol suitable for subsequent quantification by liquid chromatography tandem mass spectrometry has been reported in, "Extraction and quantitative determination of bile acids in feces" [1]. The data highlight the importance of optimization of the extraction procedure and the stability of the bile acids in feces post-extraction and prior to analysis and after several freeze-thaw cycles.
测量粪便中的胆汁酸在疾病预防、诊断和治疗中具有重要作用，并且可被视为健康状况的一种衡量指标。因此，主要目的是开发一种灵敏、稳健且高通量的液相色谱串联质谱法，该方法所需样品前处理最少，用于定量测定适用于大型队列研究的人类粪便中的胆汁酸。由于胆汁酸的化学多样性、它们在粪便中的浓度范围广泛以及粪便本身的复杂性，开发一种针对胆汁酸的灵敏且选择性的分析方法具有挑战性。在《粪便中胆汁酸的提取与定量测定》[1]中报道了一种使用甲醇的简单提取方法，该方法适用于随后通过液相色谱串联质谱法进行定量分析。数据突出了优化提取程序以及胆汁酸在提取后、分析前以及经过多次冻融循环后的稳定性的重要性。
