GC-HRTOF-MS dataset of metabolites extracted from sorghum and (a fermented product) produced using two strains of (singly and in combination).

This data article reports the untargeted metabolite profile of whole grain sorghum ( L.) and fermented samples obtained using two strains of . The sorghum grains were obtained from Agricol Johannesburg (South Africa) and fermentation was done at 34 °C for 24 h. Controlled fermentation with two strains (L. FUA 3165 and L. FUA 3321), was done using the strains singly and in combination. The samples obtained thereafter were freeze-dried and acetonitrile/methanol/water (v/v/v) were used as extraction solvent, before analyses on a gas chromatography high resolution time of flight mass spectrometry (GC-HRTOF-MS) system. Data obtained showed the presence of different compounds, classified into metabolite groups such as acids, alcohols, benzenes, furan, esters, hydrocarbons, terpenes, phytosterols, etc., with their retention time, molecular formula, observed mass and average peak areas reported herein. These data can be used for finding biomarkers for sorghum and their derived fermented products.