Central Lab, The Fifth People's Hospital of Suzhou, Suzhou, China.
The Fifth People's Hospital of Suzhou, Suzhou, China.
J Clin Lab Anal. 2021 Jul;35(7):e23831. doi: 10.1002/jcla.23831. Epub 2021 May 24.
Negative regulatory T cells (Tregs) not only deplete effector T cells but also inhibit the clearance of HIV during infection, which may allow Tregs to be used as informative diagnostic markers. To facilitate both diagnosis and treatment, a thorough understanding of these regulators by characterizing them on temporal and spatial scales is strongly required.
Hundred HIV-infected/AIDS patients, including 87 males, with an average age of 35.8 years, as well as 20 healthy controls, were enrolled. Flow cytometry was used to analyze CD3+T cells, CD4+T cells, and CD8+T cells to evaluate the immune status of the participants. Then, a group of representative negative regulatory T cells, including CD4+PD-1+T cells, CD4+PD-1 T cells, CD8+PD-1+T cells, and CD4+CD25 Tregs was also analyzed to explore their effects on disease progression and intercorrelation.
The percentages of CD4 PD-1 T cells and CD4 CD25 Tregs increased in patients with the same ultrahigh significance. Temporally, the patients with both intermediate-stage and late-stage disease had higher percentages of CD4 PD-1 T cells; however, the percentage of CD4 CD25 Tregs only increased in the patients with late-stage disease. In addition, CD4 PD-1 T cells but not CD4 CD25 Tregs were negatively correlated with the absolute CD4 T cell count. Spatially, no correlations between CD4 PD-1 T cells and CD4 CD25 Tregs were observed, which suggests these Tregs function differently during immunosuppression.
This study characterized negative regulatory T cells in HIV-infected/AIDS patients at both temporal and spatial scales and found that CD4 CD25 Tregs and CD4 PD-1 T cells could be used as potential diagnostic markers for identifying different disease stages and monitoring disease progression.
负性调节 T 细胞（Tregs）不仅耗竭效应 T 细胞，而且在感染期间抑制 HIV 的清除，这可能使 Tregs 被用作有意义的诊断标志物。为了便于诊断和治疗，强烈需要通过在时间和空间尺度上对这些调节剂进行特征描述来充分了解它们。
纳入 100 名 HIV 感染者/艾滋病患者，包括 87 名男性，平均年龄 35.8 岁，以及 20 名健康对照者。采用流式细胞术分析 CD3+T 细胞、CD4+T 细胞和 CD8+T 细胞，以评估参与者的免疫状态。然后，分析了一组有代表性的负性调节 T 细胞，包括 CD4+PD-1+T 细胞、CD4+PD-1 T 细胞、CD8+PD-1+T 细胞和 CD4+CD25 Tregs，以探讨它们对疾病进展和相互关系的影响。
在相同超高意义上，CD4 PD-1 T 细胞和 CD4 CD25 Tregs 的百分比增加。从时间上看，中期和晚期疾病患者的 CD4 PD-1 T 细胞百分比较高；然而，只有晚期疾病患者的 CD4 CD25 Tregs 百分比增加。此外，CD4 PD-1 T 细胞而不是 CD4 CD25 Tregs 与绝对 CD4 T 细胞计数呈负相关。从空间上看，CD4 PD-1 T 细胞与 CD4 CD25 Tregs 之间没有相关性，这表明这些 Tregs 在免疫抑制期间的功能不同。
本研究在时间和空间尺度上对 HIV 感染者/艾滋病患者的负性调节 T 细胞进行了特征描述，发现 CD4 CD25 Tregs 和 CD4 PD-1 T 细胞可作为识别不同疾病阶段和监测疾病进展的潜在诊断标志物。
