Magdy Selim Ahmed, Atwa Samar Magdy, El Gedawy Attia Abdullah, Younis Emad Elsayed
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Bacteriology Department, Animal Health Research Institute, Giza, Egypt.
Anim Biotechnol. 2022 Dec;33(7):1655-1660. doi: 10.1080/10495398.2021.1928683. Epub 2021 May 24.
Caseous lymphadenitis (CLA) is a chronic and insidious disease that mainly affects small ruminants and caused by (). The aims of this research were to identify by PCR from pyogenic lesions, to study the phylogenetic analysis of and to detect the prevalence based on the detected superficial lesions of CLA in Dakahlia governorate, Egypt. Out of 3471 clinically examined animals, 129 (3.71%) animals were affected with CLA. The isolation rate of in abscess of sheep was 45.74% (59/129). Out of 129 samples examined by PCR assay, 63 (48.83%) were positive phospholipase D (PLD) indicated at fragment size 203 bp. This is the first phylogenetic analysis study of isolate in Egypt which was isolated from infected sheep. Nucleotide sequence identity data demonstrated that PLD gene (MW187942) Dakahlia share homology 99.01%, 98.83 and 98.48% with Zagazig, Egypt (MN867024), Tamil nadu, India (MG720636) and Sudan (MG692441), respectively. In conclusion, this study provided information on the molecular detection and phylogeny of in Egypt. Findings of this study can be conducted in other CLA endemic countries with similar animal breeding practices in the Middle East and developing countries.
干酪性淋巴结炎（CLA）是一种主要影响小型反刍动物的慢性隐匿性疾病，由（）引起。本研究的目的是通过聚合酶链反应（PCR）从化脓性病变中鉴定（），对（）进行系统发育分析，并根据埃及达卡利亚省检测到的CLA浅表病变来检测其患病率。在3471只接受临床检查的动物中，129只（3.71%）动物感染了CLA。绵羊脓肿中（）的分离率为45.74%（59/129）。在通过PCR检测的129个样本中，63个（48.83%）在203 bp片段大小处显示磷脂酶D（PLD）呈阳性。这是埃及首次对从感染绵羊中分离出的（）进行系统发育分析研究。核苷酸序列同一性数据表明，达卡利亚的（）PLD基因（MW187942）与埃及扎加齐格（MN867024）、印度泰米尔纳德邦（MG720636）和苏丹（MG692441）的同源性分别为99.01%、98.83%和98.48%。总之，本研究提供了埃及（）的分子检测和系统发育信息。本研究结果可在中东和发展中国家其他具有类似动物养殖方式的CLA流行国家开展。
