Department of Surgery, Medical University of South Carolina.
Georgetown University;
J Vis Exp. 2021 May 10(171). doi: 10.3791/62530.
Although the liver is currently accepted as the primary transplantation site for human islets in clinical settings, islets are transplanted under the kidney capsule in most rodent preclinical islet transplantation studies. This model is commonly used because murine intrahepatic islet transplantation is technically challenging, and a high percentage of mice could die from surgical complications, especially bleeding from the injection site post-transplantation. In this study, two procedures that can minimize the incidence of post-infusion portal vein bleeding are demonstrated. The first method applies an absorbable hemostatic gelatin sponge to the injection site, and the second method involves penetrating the islet injection needle through the fat tissue first and then into the portal vein by using the fat tissue as a physical barrier to stop bleeding. Both methods could effectively prevent bleeding-induced mouse death. The whole liver section showing islet distribution and evidence of islet thrombosis post-transplantation, a typical feature for intrahepatic islet transplantation, were presented. These improved protocols refine the intrahepatic islet transplantation procedures and may help laboratories set up the procedure to study islet survival and function in pre-clinical settings.
尽管肝脏目前被认为是临床环境中人类胰岛移植的主要部位，但在大多数啮齿动物临床前胰岛移植研究中，胰岛是被移植到肾脏包膜下的。这种模型通常被使用，因为鼠类肝内胰岛移植技术上具有挑战性，而且很大比例的小鼠可能会因手术并发症而死亡，尤其是移植后注射部位出血。在这项研究中，展示了两种可以最小化输注后门静脉出血发生率的程序。第一种方法是将可吸收止血明胶海绵应用于注射部位，第二种方法是先用胰岛注射针穿透脂肪组织，然后将其作为物理屏障进入门静脉以阻止出血。这两种方法都可以有效地防止出血引起的小鼠死亡。还展示了整个肝段显示胰岛分布和移植后胰岛血栓形成的证据，这是肝内胰岛移植的一个典型特征。这些改进的方案完善了肝内胰岛移植程序，可能有助于实验室建立该程序以研究临床前环境中的胰岛存活和功能。
