Danish Research Institute of Translational Neuroscience (DANDRITE), Department of Biomedicine, Aarhus University; Universitätsklinikum Würzburg, Institut für Klinische Neurobiologie.
Danish Research Institute of Translational Neuroscience (DANDRITE), Department of Biomedicine, Aarhus University; Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University;
J Vis Exp. 2021 May 6(171). doi: 10.3791/62458.
Object place recognition is a prominent method used to investigate spatial memory in rodents. This object place recognition memory forms the basis of the object location task. This paper provides an extensive protocol to guide the establishment of an object location task with the option of up to four repetitions using the same cohort of rats. Both weak and strong encoding protocols can be used to study short- and long-term spatial memories of varying strength and to enable the implementation of relevant memory-inhibiting or -enhancing manipulations. In addition, repetition of the test with the counterbalancing presented here allows the combination of results from two or more tests for within-subject comparison to reduce variability between rats. This method helps to increase statistical power and is strongly recommended, particularly when running experiments that produce high variation in individual behavior. Finally, implementation of the repeated object location task increases the efficiency of studies that involve surgical procedures by saving time and labor.
物体位置识别是一种用于研究啮齿动物空间记忆的突出方法。这种物体位置识别记忆构成了物体位置任务的基础。本文提供了一个广泛的方案，指导使用相同的大鼠队列进行多达四次重复的物体位置任务的建立。可以使用弱和强编码方案来研究短期和长期的不同强度的空间记忆，并能够实施相关的记忆抑制或增强操作。此外，通过这里呈现的平衡重复测试，允许将来自两个或更多测试的结果进行组合，以便进行个体内比较，从而减少大鼠之间的变异性。这种方法有助于提高统计功效，强烈建议使用，特别是在运行个体行为变化较大的实验时。最后，重复物体位置任务的实施通过节省时间和劳动力来提高涉及手术程序的研究的效率。
