Muñoz Aitor, Pillot Jean-Paul, Cubas Pilar, Rameau Catherine
Centro Nacional de Biotecnología-CSIC, Madrid, Spain.
Université Paris-Saclay, INRAE, AgroParisTech, Institut Jean-Pierre Bourgin, Versailles, France.
Methods Mol Biol. 2021;2309:115-127. doi: 10.1007/978-1-0716-1429-7_10.
Shoot branching is a highly variable trait that evolves during plant development and is influenced by environmental and endogenous cues such as hormones. In particular, strigolactones (SLs) are hormones that play a key role in the control of shoot branching. Branch primordia, axillary buds formed in the leaf axils, display differential growth depending on their position in the plant and also respond to hormone signaling. In this chapter, we will describe how to quantify the degree of shoot branching in two plant model species, Arabidopsis and pea, commonly used to decipher the control of this complex trait. We will also propose several methods to perform treatments of SL or SL analogs, to investigate their bioactivity and effect on the shoot branching patterns of plants of different genotypes.
shoot分支是一种高度可变的性状，在植物发育过程中演变，并受环境和内源性信号（如激素）影响。特别是，独脚金内酯（SLs）是在控制 shoot分支中起关键作用的激素。分支原基，即在叶腋中形成的腋芽，根据其在植物中的位置表现出不同的生长，并且也对激素信号作出反应。在本章中，我们将描述如何量化两种常用于解读这种复杂性状控制机制的植物模式物种——拟南芥和豌豆的 shoot分支程度。我们还将提出几种进行SL或SL类似物处理的方法，以研究它们的生物活性以及对不同基因型植物的 shoot分支模式的影响。
