Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA.
Methods Mol Biol. 2021;2304:1-35. doi: 10.1007/978-1-0716-1402-0_1.
Over the last 30 years, confocal microscopy has emerged as a primary tool for biological investigation across many disciplines. The simplicity of use and widespread accessibility of confocal microscopy ensure that it will have a prominent place in biological imaging for many years to come, even with the recent advances in light sheet and field synthesis microscopy. Since these more advanced technologies still require significant expertise to effectively implement and carry through to analysis, confocal microscopy-based approaches still remain the easiest way for biologists with minimal imaging experience to address fundamental questions about how their systems are arranged through space and time. In this review, we discuss a number of advanced applications of confocal microscopy for probing the spatiotemporal dynamics of biological systems.
在过去的 30 年中，共聚焦显微镜已成为许多学科中生物学研究的主要工具。共聚焦显微镜的使用简单且广泛普及，确保了即使在近年来的光片和场合成显微镜取得了进展的情况下，它在生物成像领域仍将占据重要地位。由于这些更先进的技术仍然需要专业知识才能有效地实施并进行分析，因此对于成像经验有限的生物学家来说，基于共聚焦显微镜的方法仍然是解决其系统在空间和时间上如何排列的基本问题的最简单方法。在这篇综述中，我们讨论了共聚焦显微镜在探测生物系统时空动态方面的一些高级应用。
