School of Biological Sciences, The University of Manchester, Manchester, UK.
Methods Mol Biol. 2021;2317:229-245. doi: 10.1007/978-1-0716-1472-3_12.
Petunia hybrida is a commercial ornamental plant and is also an important model species for genetic analysis and transgenic research. Here we describe the steps required to isolate stable plastid transformants in P. hybrida using the commercial Pink Wave cultivar. Wave cultivars are popular spreading Petunias sold as ground cover and potted plants. Transgenes introduced into P. hybrida plastids exhibit stable expression over many generations. The development of plastid transformation in P. hybrida provides an enabling technology to bring the benefits of plastid engineering, including maternal inheritance and stable expression of performance-enhancing trait genes, to the important floriculture and horticulture industries.
矮牵牛是一种商业观赏植物，也是遗传分析和转基因研究的重要模式物种。在这里，我们描述了使用商业品种“粉红波浪”分离矮牵牛稳定质体转化体所需的步骤。波浪品种是作为地被植物和盆栽植物出售的流行的蔓生矮牵牛。导入矮牵牛质体的转基因在许多代中表现出稳定的表达。矮牵牛质体转化的发展为利用质体工程带来的益处提供了一种可行的技术，包括母系遗传和增强性能的基因的稳定表达，这对重要的花卉和园艺产业具有重要意义。
