Ruf Stephanie, Bock Ralph
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany.
Methods Mol Biol. 2021;2317:217-228. doi: 10.1007/978-1-0716-1472-3_11.
Tomato (Solanum lycopersicum L.), a member of the nightshade family (Solanaceae), is one of the most important vegetable crops and has long been an important model species in plant biology. Plastid biology in tomato is especially interesting due to the chloroplast-to-chromoplast conversion occurring during fruit ripening. Moreover, as tomato represents a major food crop with a fleshy fruit that can be eaten raw, the development of a plastid transformation protocol for tomato was of particular interest to plant biotechnologists. Recent methodological improvements have made tomato plastid transformation more efficient, and facilitated applications in metabolic engineering and molecular farming. This chapter describes the basic methods involved in the generation and analysis of tomato plants with transgenic chloroplast genomes and summarizes recent applications of tomato plastid transformation in plant biotechnology.
番茄（Solanum lycopersicum L.）是茄科（Solanaceae）的一员，是最重要的蔬菜作物之一，长期以来一直是植物生物学中的重要模式物种。由于果实成熟过程中发生叶绿体向有色体的转变，番茄中的质体生物学尤其有趣。此外，由于番茄是一种主要的粮食作物，其肉质果实可生食，因此开发番茄质体转化方案对植物生物技术学家特别有吸引力。最近方法上的改进使番茄质体转化更高效，并促进了其在代谢工程和分子农业中的应用。本章描述了具有转基因叶绿体基因组的番茄植株的产生和分析所涉及的基本方法，并总结了番茄质体转化在植物生物技术中的最新应用。
