State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.
Plant Physiol Biochem. 2021 Aug;165:1-9. doi: 10.1016/j.plaphy.2021.05.016. Epub 2021 May 14.
Stress associated proteins (SAPs), a class of A20/AN1 zinc finger domain-containing proteins, are involved in a variety of biotic and abiotic stress responses in plants. However, little is known about the SAP gene family and their functions in Tamarix hispida. In this study, we isolated and characterized 11 SAPs from T. hispida. The expression patterns of ThSAPs were analyzed under various stresses (salt and drought) and phytohormone treatment (SA, ABA and MeJA) using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Most ThSAPs exhibited transcriptional responses to abiotic stresses and phytohormones. Among these ThSAPs, ThSAP6 was significantly induced by salt stress. Gain-and loss-of-function analyses revealed that ThSAP6 was a positive regulator of salt stress response. Overexpression of ThSAP6 in T. hispida increased antioxidant enzymes activity and proline content and decreased reactive oxygen species (ROS) accumulation and cell membrane damage under salt stress, while the opposite physiological changes were observed in ThSAP6-RNAi (RNA interference) lines. This study provides a comprehensive description of the SAP gene family in T. hispida, and demonstrates that ThSAP6 is a potential candidate for biotechnological approaches to improve salt tolerance in plants.
应激相关蛋白（SAPs）是一类含有 A20/AN1 锌指结构域的蛋白质，参与植物的多种生物和非生物胁迫反应。然而，关于 SAP 基因家族及其在柽柳中的功能知之甚少。本研究从柽柳中分离并鉴定了 11 个 SAPs。采用实时定量反转录聚合酶链反应（RT-qPCR）分析了 ThSAPs 在各种胁迫（盐和干旱）和植物激素处理（SA、ABA 和 MeJA）下的表达模式。大多数 ThSAPs 对非生物胁迫和植物激素表现出转录响应。在这些 ThSAPs 中，ThSAP6 被盐胁迫显著诱导。功能获得和丧失分析表明，ThSAP6 是盐胁迫反应的正调控因子。在盐胁迫下，柽柳中超表达 ThSAP6 增加了抗氧化酶活性和脯氨酸含量，减少了活性氧（ROS）积累和细胞膜损伤，而在 ThSAP6-RNAi（RNA 干扰）系中观察到相反的生理变化。本研究全面描述了柽柳中的 SAP 基因家族，并表明 ThSAP6 是生物技术提高植物耐盐性的潜在候选基因。
