Identification and Analysis of Stress-Associated Protein (SAP) Transcription Factor Family Members in .

Stress-associated proteins (SAPs), belonging to the A20/AN1 zinc finger protein family, are key regulators in plant stress responses. Despite their importance, studies on the SAP gene family in are still relatively scarce. This study aimed to systematically identify and characterize SAP genes in and to explore their potential roles in stress response mechanisms. A total of 17 genes were identified from . Phylogenetic analysis revealed that these genes group into five distinct clades, and 10 conserved motifs were identified. Using transcriptome data and qRT-PCR, we analyzed their expression patterns and employed yeast systems to validate their transcriptional activities. The responses of gene family members to different stress treatments showed significant differences. For example, and responded strongly to ABA, MeJA, and HO treatments, while and showed significant upregulation under ETH and NaCl stress. Yeast experiments indicated that were transcriptional activators, and and were transcriptional suppressors. The identification and preliminary analysis of genes provided a theoretical foundation for understanding stress resistance mechanisms in