蛹虫草来源真菌拟青霉改善实验性自身免疫性甲状腺炎小鼠的病情。
Cordyceps sinensis-derived fungus Isaria felina ameliorates experimental autoimmune thyroiditis in mice.
机构信息
Affiliated Cancer Hospital, Shanxi Medical University, Taiyuan 030013, China; Laboratory Animal Center, Shanxi Cancer Institute, Taiyuan 030013, China.
Affiliated Cancer Hospital, Shanxi Medical University, Taiyuan 030013, China; Laboratory Animal Center, Shanxi Cancer Institute, Taiyuan 030013, China.
出版信息
Biomed Pharmacother. 2021 Aug;140:111733. doi: 10.1016/j.biopha.2021.111733. Epub 2021 May 21.
AIMS
This study aimed to investigate the therapeutic effect of Cordyceps sinensis-derived fungus Isaria felina on experimental autoimmune thyroiditis (EAT).
METHODS
A NaI-induced EAT mouse model was established. The mice received oral administration of vehicle, low-dose Isaria felina (300 mg/kg), or high-dose Isaria felina (600 mg/kg) once a day for four weeks before euthanasia. Enzyme-linked immunosorbent assays (ELISA) was performed to measure serum thyroid-stimulating hormone (TSH) levels, thyroid antibodies, and cytokines. Hematoxylin and eosin (H&E) staining was conducted to assess histopathological changes in the thyroid tissue samples of mice. TUNEL and Bcl-2 immunohistochemistry (IHC) were performed to evaluate cell apoptosis, and cleaved caspase-3 IHC was performed to detect the relative expression in the thyroid tissue samples.
RESULTS
Compared with KIO and KI water, NaI water consumption successfully induced EAT in mice, as evidenced by significantly increased circulating TSH and thyroid antibody levels, along with typical histopathological abnormalities of autoimmune thyroiditis (AIT) in the thyroid tissue samples. Compared with vehicle or low-dose Isaria felina, high-dose Isaria felina treatment resulted in significant reductions in white cell counts and circulating TSH, thyroid antibody, and cytokine levels of EAT mice. High-dose Isaria felina also alleviated histopathological abnormalities and attenuated TUNEL staining, Bcl-2 protein expression, and cleaved caspase-3 expression in the thyroid tissue samples.
CONCLUSION
High-dose Isaria felina treatment alleviates thyroid inflammation and cell apoptosis in EAT, serving as a novel, promising therapeutic agent for AIT.
目的
本研究旨在探讨蛹虫草衍生真菌蝉拟青霉对实验性自身免疫性甲状腺炎（EAT）的治疗作用。
方法
建立碘化钠诱导的 EAT 小鼠模型。在处死前，小鼠每天口服给予 vehicle、低剂量蝉拟青霉（300mg/kg）或高剂量蝉拟青霉（600mg/kg），连续 4 周。采用酶联免疫吸附试验（ELISA）检测血清促甲状腺激素（TSH）水平、甲状腺抗体和细胞因子。对小鼠甲状腺组织进行苏木精和伊红（H&E）染色，评估组织病理学变化。采用 TUNEL 和 Bcl-2 免疫组化（IHC）评估细胞凋亡，采用 cleaved caspase-3 IHC 检测甲状腺组织中的相对表达。
结果
与 KIO 和 KI 水相比，NaI 水的摄入成功诱导了小鼠的 EAT，表现为循环 TSH 和甲状腺抗体水平显著升高，甲状腺组织出现典型的自身免疫性甲状腺炎（AIT）组织病理学异常。与 vehicle 或低剂量蝉拟青霉相比，高剂量蝉拟青霉治疗导致 EAT 小鼠的白细胞计数和循环 TSH、甲状腺抗体和细胞因子水平显著降低。高剂量蝉拟青霉还缓解了甲状腺组织的组织病理学异常，减少了 TUNEL 染色、Bcl-2 蛋白表达和 cleaved caspase-3 表达。
结论
高剂量蝉拟青霉治疗可减轻 EAT 中的甲状腺炎症和细胞凋亡，为 AIT 提供了一种新的、有前途的治疗药物。
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